银屑病抗体搭桥角朊细胞/单一核细胞粘连:白介素2及白介素6和白介素2受体α链的检测  

Psoriatic Antibody Bridging Keratinocyte/Mononuclear Leucocyte Adhesion: Estimation of IL 2, IL 6 and IL 2Rα

作　　者：胡长发[1] 管晓春[1] 闫鸣[1] 汪汉超 冯永芳[1] 

机构地区：[1]武汉市第一医院皮肤科,430022

出　　处：《中华皮肤科杂志》1996年第2期96-98,共3页Chinese Journal of Dermatology

基　　金：国家自然科学基金

摘　　要：抗体搭桥角朊细胞／单一核细胞粘连（ＡＢＫＭＡ）作为银屑病研究一个体外实验模型，用来研究银屑病的免疫激活的细胞因子网改变。正常人表皮细胞悬液分离自包皮，加入银屑病ＩｇＧ孵育后加入银屑病单一核细胞，形成ＡＢＫＭＡ。使用ＡＢＣ增强的ＥＬＩＳＡ检测培养上清的白介素２和白介素６。实验结果表明，白介素２和白介素６的合成和分泌都高于正常人ＩｇＧ对照和无血清培养基对照。细胞表面的白介素２受体α链（ＩＬ２Ｒα）用免疫组织化学染色法显示。ＩＬ２Ｒα＋单一核细胞／单一核细胞为４．６％。ＩＬ２Ｒα单一核细胞／粘连的单一核细胞为４０．４％，这两者都高于相应的对照。Antibody bridging keratinocyte/mononuclear leucocyte adhesion(ABKMA), an experimental model in vitro for psoriasis, has been used in the investigation of cytokine network changes. ABKMA was formed by using normal human epidermal cells incubated with IgG of patients with psoriasis, and then mononuclear leucocytes(ML) of patients with psoriasis were added. Interleukin 2 and interleukin 6 in culture supernate were estimated by ABC amplified ELISA. The results indicated that the synthesis and secretion of interleukin 2 and interleukin 6 were higher than normal IgG and serum free medium controls. Immunohistochemistry staining was used to demonstrate the cell surface interleukin 2 receptor α chain (IL2Rα). The expression of IL2Rα +ML/ML and IL2Rα +ML/adhesion ML was 4.6% and 40.4% respectively. Both these data were higher than corresponding controls.

关 键 词：银屑病 ABKMA IL2 IL6 受体 检测 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]