胰岛淀粉样多肽与胰岛素在胎鼠胰腺共存的免疫组织化学研究  

IMMUNOHISTOCHEMICAL STUDY ON THE CO-EXISTENCE OF IAPP AND INS IN RAT FETAL PANCREAS

作　　者：李占淳[1] 梁文妹[1] 石爱荣[1] 

机构地区：[1]北京医科大学组织学与胚胎学系,瑞典Linkoping大学医学院病理学系

出　　处：《解剖学报》1996年第2期192-194,共3页Acta Anatomica Sinica

摘　　要：用免疫组织化学ＰＡＰ法，对第１４～２１ｄ大鼠胚胎胰腺ＩＡＰＰ和Ｉｎｓ－ＩＲ细胞进行定位研究。结果显示，胚胎第１４ｄ胰腺内可见少数散在的Ｉｎｓ－ＩＲ细胞，未见ＩＡＰＰ－ＩＲ细胞；自胚胎第１６ｄ始，可见ＩＡＰＰ－ＩＲ细胞。经邻片比较证明，ＩＡＰＰ与Ｉｎｓ共存于胰岛Ｂ细胞内；随胎龄增长，ＩＡＰＰ和Ｉｎｓ－ＩＲ细胞相伴发育；同时在胰导管上皮细胞之间，出观察到共存的ＩＡＰＰ和Ｉｎｓ－ＩＲ细胞。本文还初步讨论了ＩＡＰＰ与Ｉｎｓ在大鼠胚胎胰腺内共存的生物学意义。The localization of IAPP -IR and Ins-IR cells in rat fetal pancreas from 14th day to 21st day of gestation were studied by immunohistochemical PAP method,The result showed that cells immunoreactive for Ins were demonstrated at the 14th day,which was scattered in a few number.From 16th day onwards IAPP-IR cells were seen in the fetal pancreas,and the colocalization of IAPP and Ins in the B cells as proved as compared with adjacent sections. IAPP-IR and Ins-IR cells developed meanwhile with age.In the same time it was observed that IAPP and Ins co-existed in epithelium of pancreatic ducts.The co-existence of IAPP and Ins in rat fetal pancreas were reported for the first time in the present study.The relationship and the biological significance of the above co-localization were discussed.

关 键 词：胰岛淀粉样多肽 胰岛素 免疫组织化学 鼠胎 胰腺 

分 类 号：Q593.9[生物学—生物化学]