生脉片对体外培养软骨细胞一氧化氮合酶的影响  

Effect of Shengmai Tablet on Chondrocyte Nitric Oxide Synthesisin Vitro

作　　者：周正新[1] 李保林 杨荣建[3] 王海彬[3] 袁浩[3] 

机构地区：[1]安徽中医学院第一附属医院,安徽合肥230031 [2]广东省广州市越秀区正骨医院 [3]广州中医药大学中医髋关节疾病治疗中心

出　　处：《安徽中医学院学报》2005年第5期37-39,共3页Journal of Anhui Traditional Chinese Medical College

基　　金：国家中医药管理局科研基金项目(95B132)

摘　　要：目的:探讨中药生脉片防治骨性关节炎的作用机制。方法:通过体外软骨细胞培养的方法,应用IL-1β诱导软骨细胞产生内源性NO,分空白对照组、生脉组、L-NAME组和IL-1β组观察生脉片对软骨细胞一氧化氮合酶(NOS)的影响。结果:生脉组和L-NAME组的NOS活性明显低于IL-1β组(P<0.01)。结论:生脉片能够抑制IL-1β诱导软骨细胞合成NOS,具有防治骨性关节炎的作用。Objective： To investigate the mechanism of Shengmai tablet, a traditional Chinese medicine, in preventing osteoarthritis. Method： the chondrocyte plates were divided into four groups： Blank control group, Shengmai group, L-NAME group and IL-1β group. The effect of Shengmai tablet on NOS by chondrocyte culture in vitro and the endogenous NO secreted by IL-1β induced chondrocytes was observed. Result： the NOS activity of Shengmai group and L-NAME group were obviously lower than that of IL-1β group （P 〈 0.01 ）. Conclusion： Shengmai tablet can inhibit NOS synthesis of chondrocytes induced by IL-1β, and can prevent and cure osteoarthritis.

关 键 词：骨性关节炎 软骨细胞 一氧化氮合酶 生脉片 

分 类 号：R285.5[医药卫生—中药学]