百草枯干预不同时间后DAT和VMAT2基因表达的时程变化  

Influence of paraquat on dopamine transporter mRNA and vesicular monoamine transporter2 mRNA in the nigrostriatum of paraquat induced Parkinsonian mice

作　　者：任今鹏[1] 任惠民[1] 蒋雨平[1] 

机构地区：[1]复旦大学附属华山医院神经内科

出　　处：《神经科学通报》2005年第5期330-334,共5页Neuroscience Bulletin

基　　金：This work was supported by the development grant(No.004119005)of the Shanghai science and technology committee.

摘　　要：目的探讨百草枯干预后黑质部纹状体通路DAT和VMAT2基因表达的时程变化。方法用口服百草枯的途径,建立小鼠帕金森病模型;应用RT-PCR技术观察小鼠口服百草枯(每日10mg/kg)不同时间后黑质部DAT和VMAT2基因表达的变化。结果从口服百草枯2周起,小鼠黑质部DAT和VMAT2的基因表达开始减少,并且随着喂食时间的延长逐渐减少。结论在百草枯致PD的早期,DAT和VMAT2的基因表达就受到损害,并且随着百草枯所致PD病程的延续,受损的程度逐渐加重,表明DAT和VMAT2均可能参与了百草枯致帕金森病的发病机制。Objective To investigate the changes of time-course of dopamine transporter （DAT） mRNA and vesicular monoamine transporter2 （VMAT2） mRNA in the substantia nigra of Parkinsonian mice induced by paraquat orally. Methods RT-PCR technique was used to detect the changes of expressions of DAT and VMAT2 mRNA in the substantia nigra of mice after paraquat taken orally for different days. Results The gene expressions of DAT and VMAT2 were decreased gradually two weeks after paraquat. Conclusion In the early stage of paraquat-induced parkinsonian mice, the expres- sions of DAT and VMAT2 mRNA were decreased, and the degree of reduced expression was more severe with the development of Parkinson disease. DAT and VMAT2 may be involved in the pathogenesis of Parkinson disease.

关 键 词：帕金森病 百草枯 多巴胺转运体 囊泡单胺转运体2 

分 类 号：R285[医药卫生—中药学]