急性黄磷中毒小鼠血清中NO、GSH、MDA及总抗氧化能力的实验研究  

Experimental Study on Nitric Oxide,Glutathione Maleic Dialdehyde and Total anti-oxidive Ability in Serum of Mice with Aoute Yellow Phosphorus Poisoning

作　　者：傅庆勇[1] 李溥[1] 

机构地区：[1]黔南民族医学高等专科学校,贵州都匀558003

出　　处：《中华综合临床医学杂志（北京）》2005年第10期22-23,共2页chinese journal of composite clinical medicine

摘　　要：目的：探讨急性黄磷中毒小鼠血清中一氧化氮（NO）还原型谷胱甘肽（GSH）、丙二醛（MDA）及总抗氧化能力的变化，及其在黄磷中毒发病机制中的作用。方法：通过急性动物实验，小鼠灌胃染毒，分为低、中、高剂量组与对照组，采用生化试剂盒测定各组小鼠血清中NO、GSH、MDA及总抗氧化能力。结果：低剂量组NO、GSH则低于对照组（p〈0．05）；各剂量组NO、GSH和总抗氧化能力随染黄磷剂量的增加而降低，MDA水平则显著增高（p〈0．01）。MDA与琥珀酸脱氢酶（SDH）、葡萄糖-6-磷酸酶（G6pD）呈负相关（p〈0．01）。结论：黄磷可引起小鼠血清中GSH、MDA和总抗氧化能力发生变化，进而影响NO合成与代谢．导致脂质过氧化的发生，提示脂质过氧化可能是黄磷中毒的指标之一。Objective：To clarify the variety and the significanc of nitric oxide（NO）, glutathione（GSH）, maleic dialdehyde（MDA） and total anti-oxidize ability in serum poisoning, Methods： Animal experiment and biochemistry agent box of NO, GSH, MDA and total anti-oxidize ability were used.Results： The contents of NO and GSH in low dose groups were higher than those in controls serum of mice（P〈0.05）, and decreased in high dose group.NO, GSH and total anti-oxidze ability in all experimental groups decreased and slowed a dose-effect manner. MDA in all groups increased significantly and showed a dose-effect manner （p〈0.01）. The MDA was negatively correlationed with sorbic dehydgenase and glucose-6-phosphate dehydgenase（p〈0.01）. Conclusion： The changes of GSH, MDA and total anti-oxidize ability could be affected by yellow phosphorus. The synthesis or metabolize of NO may be correlated with the influence of yellow phosphorus on GSH, MDA and total anti-oxidize ability. The results induce lipid peroxidation （LPO） in mice, LPO might be one of the mechanism of yellow phosphorus poisoning.

关 键 词：黄磷 一氧化氮 谷胱甘肽 丙二醛 脂质过氧化 丙二醛(MDA) 总抗氧化能力 急性动物实验 黄磷中毒 小鼠血清 

分 类 号：R595[医药卫生—内科学] R-33[医药卫生—临床医学]