酶浸提取方法对士的宁产率的影响  

Effect of Different Extraction Technology on Yield of Strychnine

作　　者：张福维[1] 孙小平[1] 关崇新[1] 辛广[1] 

机构地区：[1]鞍山师范学院化学系,鞍山114005

出　　处：《生物技术》2005年第5期62-63,共2页Biotechnology

摘　　要：目的:研究了纤维素酶在提取生物碱过程中的应用。方法:采用酶浸法和氯仿法两种不同的工艺提取马钱子生物总碱,高效液相色谱法(HPLC)测定了马钱子生物总碱中士的宁的含量。结果:酶浸法提取士的宁和氯仿法提取士的宁的含量分别为1.83%、1.32%;酶浸法和氯仿法提取马钱子生物总碱的产率分别为:2.85%、1.86%。Objective：To study the cellulose enzyme in treating alkaloid to increase the yield of the strychnine.Methods：Using method of enzyme leaching and method of choloroform extraction teclmology to extract strychnine of strychnosmax - vomica. 1, the contents of strychnine were determihated by HPLC. Results： The content of strychnine extracted by method of enzyme leaching was 1.83 %, the content of strychnine extracted by method of choloroform was 1.32% .The contents of total alkaloids extracted by two methods were 2.85% and 1.86 respectively.

关 键 词：酶浸法 氯仿法 HPLC法 含量测定 

分 类 号：R284.2[医药卫生—中药学]