刺激中脑防御反应区诱导延髓PGL、NRO的c－Fos表达  

THE EXPRESSION OF c-Fos IN NUCLEUS RAPHE OBSCURUS AND PARAGIGANTOCELLULARIS LATERALIS FOLLOWING STIMULATION OF DORSAL PERIAQUEDUCTAL GRAY

作　　者：黄建华[1] 龚茜玲[1] 李鹏[1] 

机构地区：[1]上海医科大学基础医学院生理学教研室,上海铁道大学医学院生理学教研室

出　　处：《上海医科大学学报》1996年第2期117-121,共5页Journal of Fudan University(Medical Science)

基　　金：国家自然科学基金

摘　　要：为从形态学上明确中缝隐核（ＮＲＯ）、巨细胞旁外侧核（ＰＧＬ）与防御反应的关系，并分析为防御性心血管活动激活的ＮＲＯ神经元的性质，本实验采用免疫细胞化学Ｆｏｓ诱导显示ＡＢＣ－ＤＡＢ法及Ｆｏｓ＋５－ＨＴ双标法，分别观察电刺激中脑导水管周围灰质背侧部（ｄＰＡＧ）、改变刺激强度或表达时间诱导的ＮＲＯ、ＰＧＬ的Ｆｏｓ样免疫阳性反应（ＦＬＩ）细胞及刺激ｄＰＡＧ诱导的ＮＲＯ的Ｆｏｓ＋５－ＨＴ双标细胞的分布。实验在戊巴比妥钠麻醉的Ｓｐｒａｇｕｅ－Ｄａｗｌｅｙ（ＳＤ）大鼠上进行。结果显示，刺激ｄＰＡＧ所诱导的ＮＲＯ、ＰＧＬ的ＦＬＩ细胞数均较对照组明显增加（Ｐ＜０．００１），并与刺激电流的强度有一定的依从关系，而刺激后间隔３ｈ灌流的Ｆｏｓ表达程度显著高于间隔１ｈ者；在刺激ｄＰＡＧ诱导中缝隐核Ｆｏｓ表达并在ＡＢＣ－ＤＡＢ法显示的基础上，再以免疫组化法作５－ＨＴ抗体标记，结果ＮＲＯ未见双标细胞，提示ＮＲＯ、ＰＧＬ神经元参与了防御性心血管活动，一定强度的刺激可能导致ＮＲＯ、ＰＧＬ内神经元功能长时程的改变；参与防御性心血管活动的ＮＲＯ神经元可能为非５－ＨＴ神经元。PURPOSE To reveal nucleus raphe obscurus(NBO) and the nucleus paragigantocellularis lateralis(PGL) involved in the defence reaction and determine the type of neurons in NRO which were activated by cardiovascular defence reaction,we studied the distribution of Fos-like immunoreactivity(FLI) cells in the NRO and the PGL and distribution of the double-stained cells for Fos protein and serotonic(5-HT) in the NRO after electrical stimulation of dorsal periaqueductal gray (dPAG).METHODS The experimente were performed in Sprague-Dawley rats anaesthetized with sodium pentobarbital.Intermittent electrical stimulation at parameters set to elicit a characteristic defensive pressor response was applied to one or neither side of dPAG for 15 min. Medulla sections were incubated for 75h in anti-Fos antibody and prepared for visualization of the reaction product using the ABC immunoperoxidase technique.After Fos-expression in the NRO induced by the stimulation of the dPAG and stained with ABC-DAB immunoreactivity,the medulla sections were immunostained with the peroxidase-antiperoxidase procedure by 5-HT antibody.RESULTS An increase in FLI was observed within the NRO and the PGL bilaterally,in comparison with the control group(P<0.001).This effect was timeand strength-dependent.No double-labelled neuron was observed in the NRO.CONCLUSIONS The results suggest that the neurons of NRO and PGL are involved in the cardiovascular defence reaction,and a fairly high intensity of stimulation can induce the long lasting functional changes of the NRO or PGL neurons.The neurons mediating defensive cardiovascular activity in the NRO may be non-serotonergic.

关 键 词：中缝隐核 PGL dPAG 防御反应 C-FOS 延髓 

分 类 号：R338.2[医药卫生—人体生理学]