环胞菌素A对建立EB病毒转化的B淋巴母细胞系的促进作用  

ENHANCIVE EFFECT OF CYCLOSPORIN A ON THE ESTABLISHMENT OF EBV-TRANSFORMED HUMAN LYMPHOBLASTOID CELL LINES

作　　者：夏蓓莉[1] 左伋 叶纹[1] 李采娟 陈秀珍[1] 

机构地区：[1]上海医科大学基础医学院医学遗传研究室

出　　处：《上海医科大学学报》1996年第2期126-127,共2页Journal of Fudan University(Medical Science)

基　　金：WHO基金

摘　　要：建立有效的Ｂ淋巴母细胞系ＥＢ病毒转化法。利用免疫抑制剂环胞菌素Ａ选择性地抑制Ｔ细胞并阻止Ｂ细胞退化的作用，提高ＥＢ病毒转化人类淋巴细胞系效率，结果：建立了３０余株人类类淋巴母细胞系总成功率达９５％，比非ＣｙＡ法提高了５０％。结论：环胞菌素Ａ的应用可有效地建立淋巴细胞系，从而为保存特殊个体（如遗传病）的基因组并开展相应的研究奠定了基础。PURPOSE To increase the effciency of in vitro transformation of human lymphocytes by Epstein-Barr virus(EBV).METHODS Cyclosporin A(CyA),an immunosuppressive agent which selectively inhibit T-lymphocytes and protect B-lymphocytes from regression,was used in the experiment.RESULTS More than 30 EBV-transformed human lymphoblastoid cell lines were established by using Cy A,The successive rate increased remarkably up to 95%,compared to 50% without using CyA.CONCLUSIONS This mothod by using cyA can be routinely used for the efficient banking of large number of lymphoblastoid cell lines.

关 键 词：环胞菌素A 细胞系 E-B病毒转化 B细胞 LCL 

分 类 号：R979.5[医药卫生—药品]