海洋真菌09-1-1-1次级代谢产物的研究  

Studies on the secondary metabolites from marine fungus 09-1-1-1

作　　者：许志勇[1] 张惠平[1] 

机构地区：[1]复旦大学药学院,上海200032

出　　处：《中国海洋药物》2005年第5期43-46,共4页Chinese Journal of Marine Drugs

摘　　要：目的从海洋真菌的代谢产物中寻找新的具有抗真菌活性的化合物。方法以稻瘟霉生物模型筛选海洋真菌,获得编号09-1-1-1的活性菌株,从其发酵液中分离活性代谢产物。结果从发酵液中分离到2个化合物,鉴定其结构为3a,12c-二氢-8-羟基-6-甲氧基-7H-呋喃[3′,2′∶4,5]呋喃[2,3-c]呫吨-7-酮(Ⅰ,sterig-matocystin)和1,3,6,8-四羟基-2-(1-羟己基)-9,10-蒽二酮(Ⅱ,averantin)。结论稻瘟霉生物模型用于筛选海洋真菌活性代谢产物成本低、快速又方便。化合物Ⅱ对109稻瘟霉菌丝的生长最小抑制浓度为1.6μg.mL-1。Objective To search for novel antifungal structures produced by marine fungus. Methods Using the Pyricularia oryzae （P. oryzaae） bioassay method to screen marine fungi led to the isolation of marine fungus 09-1-1-1. Active metabolites were isolated from the broth of the marine fungus 09-1-1-1. Results Two compounds were extracted from the mycelium and their structures were elucidated by various spectroscopic methods as 3a, 12c-dihydro- 8-hydroxy-6-methoxy-7H-furo[3 , 2 ： 4,5] furo[-2,3-c] xanthen-7-one （ Ⅰ , sterigmatocystin） and 1,3,6,8-tetrahydroxy-2-（1-hydroxyhexyl） anthraquinone （ Ⅱ , averantin）. Conclusion The P. oryzae bioassay was a cheap, quick and convenient method for the screening of bioactive substances from marine fungi. Compound Ⅱ showed potent inhibitory activity to the mycelia of P. oryzae. The MIC of compound Ⅱ against P. oryzae was 1.6μg · mL^-1.

关 键 词：海洋真菌 稻瘟霉活性 1 3 6 8-四羟基-2-(1-羟己基)-9 10-蒽二酮 

分 类 号：Q949.320.6[生物学—植物学]