维A酸致大鼠骨质疏松模型与机理研究  

A MODEL OF OSTEOPOROSIS INDUCED BY KETINOIC ACID IN MALE WISTAR RATS

作　　者：吴波[1] 徐冰[1] 黄添友[1] 王金锐 

机构地区：[1]深圳三九集团医药研究院药理室

出　　处：《药学学报》1996年第4期241-245,共5页Acta Pharmaceutica Sinica

摘　　要：３月龄雄性Ｗｉｓｔａｒ大鼠连续ｐｏ维Ａ酸７０ｍｇ·ｋｇ－１·ｄ－１共１４小成功地建立了骨质疏松模型。胜骨组织形态计量学检测结果显示，模型大鼠松质骨和密质骨骨量明显丢失，与对照鼠相比，ｐ＜０．０５。模型大鼠骨组织形态发生显著病理改变，胜骨骨小梁密度减少、间隙增大，胫骨中段骨皮质变薄、骨髓腔扩大。反映骨细胞活性与功能的各项参数变化表明，维Ａ酸对类骨质形成和骨基质钙化无明显影响，但使破骨细胞活性与功能显著增强，从而促进骨吸收，使骨代谢处于骨吸收大于骨形成的负平衡状态，导致大鼠发生骨质疏松症。实验还观察到模型大鼠体重减轻，前列腺和精囊重量减少，肾上腺和脾脏肥大，但血中性激素和反映骨代谢状况的生化指标Ｃａ，Ｐ和ＡＬＰ无明显改变。An animal model of osteoporosis induced by retinoic acid was successfully established in 3-month-old male Wistar rats.The animals were given the drug 70 mg·kg-1·d-1for14 d intragastrically and sacrificed on day 29.The proximal tibia and middle tibia of the rats wereprocessed undecalcifiedly for quantitative bone histomorphometry. Compared with the control rats,thecancellous and compact bone volume of the model rats were reduced markedly.The bone tissuemicrostructure showed some obvious pathological changes that the trabecular number were decreased,the separation of trabecular and medulla ossium cavity became large,the thickness of trabecular andcortex of bone were decreased.Themechanism of bone loss in the model rats was that the osteoclast was activated by retinoic acid which promoted bone resorption,Other changes in the model rats werealso observed such as:the body weight,and the weights of seminal vesicle and prostate weredecreased, the adrenal glands and spleen showed hyperplasia and hypertrophy.No change of the bloodconcentration of calcium,phosphorus,alkaline phosphatase,alanine transaminase,estradiol andtestosterone in the model rats was observed.

关 键 词：维A酸 骨质疏松 药物副作用 

分 类 号：R977.21[医药卫生—药品] R681[医药卫生—药学]