罗氏沼虾血清及肌肉提取液凝集活性的比较研究  

Comparative Studies on Hemagglutinating Activity of Serum and Muscular Extract From Macrobrachium rosenbergi

作　　者：戴聪杰[1] 陈寅山[2] 谢进金[1] 吴文林[1] 

机构地区：[1]泉州师范学院生物系,福建泉州362000 [2]福建师范大学生物工程学院,福建福州350007

出　　处：《泉州师范学院学报》2005年第2期68-72,共5页Journal of Quanzhou Normal University

基　　金：福建省自然科学基金资助项目(B0410027);泉州科技局重点科技项目(Z2002-39)

摘　　要：用12种天然或经酶修饰的动物及人红细胞测定罗氏沼虾的血清和肌肉提取液的凝集能力,同时进行糖抑制、热稳定性、pH及Ca2+影响试验.结果显示,罗氏沼虾血清对鹌鹑红细胞的凝集效价比肌肉提取的高,且糖抑制、热稳定性、pH适应范围及对Ca2+的依赖性都存在明显差别,表明罗氏沼虾血清和肌肉提取液所含凝集素的类型不同.Serum and muscular extract from Macrobrachium rosenbergi are tected for hemag- glutinins using 12 natural and enzyme-treated erythrocytes of human and animal, besides, glyco-inhibiting,thermostability and influence of pH and Ca^2＋ to the activity of them are tested. The results show that the agglutination titer of serum is higher than that of muscular extract from Macrobrachium rosenbergi, and, there are evident difference in glyco-inhibiting, thermostability,range of pH and the required of Ca^2＋ between serum and muscular extract. These results indicate that both of serum and muscle of Macrobrachium rosenbergi contain lectin,and they are different in nature.

关 键 词：罗氏沼虾 血清 肌肉提取液 凝集素 

分 类 号：Q959.233[生物学—动物学]