毛细管电泳电化学检测法测定连翘中的芦丁  

Determination of Rutin in Forsythia suspense(Thunb.) Vahl by Capillary Electrophoresis with Electrochemical Detection

作　　者：彭友元[1] 

机构地区：[1]泉州师范学院化学系,福建泉州362000

出　　处：《泉州师范学院学报》2005年第4期47-50,共4页Journal of Quanzhou Normal University

基　　金：福建省青年创新基金重点项目(2003J037)

摘　　要：采用毛细管电泳电化学检测法测定了连翘中的芦丁.考察了工作电极的氧化电位、分离电压和进样时间对分离和检测的影响.在优化条件下,以300μm直径的碳圆盘电极为检测电极,检测电位为+0.9 V(vs.SCE),在50 mmol/L硼酸盐(pH8.4)的运行缓冲液中,被测物浓度与峰电流在三个数量级范围内呈良好线性,检测限为2×10-7g/mL.方法有着良好的重现性,被测组分的迁移时间和峰高的相对标准偏差(RSDs)小于4%(n=7).该法简单、可靠、快速,单次测定可在16 min完成,已经成功的应用于实际样品中芦丁的测定,样品处理简单,无须预富集,检测结果令人满意.The determination of rutin in Forsythia suspense （Thunb.） Vahl by capillary electrophoresis with electrochemical detection （CE-ED） was reported. Effects of several important factors such as separation voltage, injection time, and detection potential were investigated to acquire the optimum conditions. Under the optimum conditions, the analyte could be separated within 16 min in a 75 cm length capillary at a separation voltage of 18 kV in a 50 mmol/L borate buffer （pH=8.4）. A 300μm diameter carbon disk electrode generates good response at ＋0.90 V （vs. SCE） for rutin. The relationship between peak current and analyte concentration was linear over about three orders of magnitude with detection limit （S/N=3） of 2 × 10^-7 g/mL for rutin. This proposed method demonstrated long-term stability and reproducibility with relative standard deviations of less than 4% for both migration time and peak current （n=7）. It has been successively used for the determination of rutin in real sampies,and the assay result was satisfactory.

关 键 词：毛细管电泳 电化学检测 连翘 芦丁 

分 类 号：O657[理学—分析化学]