基于Nafion/双层L-半胱氨酸-纳米金固定辣根过氧化氢酶的生物传感器  

Hydrogen peroxide biosensor with HRP immobilized on a layer-by-layered L-cysteine/Au film with Nation as a metrix

作　　者：黎雪莲[1] 袁若[1] 柴雅琴[1] 朱强[1] 王娜[1] 

机构地区：[1]西南大学化学化工学院重庆市现代分析化学重点实验室,重庆400715

出　　处：《化学传感器》2005年第3期21-25,共5页Chemical Sensors

基　　金：西南大学高新技术培育基金(XSGX02);西南师范大学博士基金(SWNUB2004021)

摘　　要：利用滴涂于金盘电极表面的 Nation 膜中负电性的磺酸基与 L-半胱氨酸阳离子之间的静电作用实现 L-半胱氨酸的固定化,然后利用 L-半胱氨酸表面的巯基吸附纳米金,荷负电的纳米金再结合 L-半胱氨酸阳离子后再吸附纳米金以制备基于多层分子自组装的辣根过氧化氢生物传感器。采用循环伏安法考察了传感器的电化学特性,用 Co(bpy)_3^(3+)作为电子媒介体,峰电流值与 H_2O_2浓度在2.5×10^(-5)～5.0×10^(-3)mol/L 成线性关系,检测下限为1.0×10^(-5)mol/L(S/N=3)。该传感器具有响应快、灵敏度高、稳定性好的性能,且具有良好的选择性,能有效排除抗坏血酸、抗尿酸等常见干扰物质对测定的影响。A novel amperometric biosensor has been developed for the determination of H2O2 based on Nation, bilayer assembled L - cystein / colloidal Au on gold disk electrode, meanwhile using Co （bpy）3^3^＋ in hydrogen peroxide solution as the mediator. Cyclic voltammetry was employed to demonstrate the feasibility of electron transfer between peroxidase and electrode. Analytical parameters such as the concentration of Nation, pH and temperature were also studied. Linear calibration for H2O2 was obtained in the range from 2.5 × 10^-5 - 5. 0 × 10^ -3 mol/L. The sensor was highly sensitive to H2O2 with a detection of 1.0 × 10^ -5 mol/L at a signal - to - noise ratio of 3. The sensor exhibited high sensitivity, stability and in the meantime the low operating potential of the biosensor can effectively eliminate interference from common interferents, such as ascorbic acid and uric acid.

关 键 词：L-半胱氨酸 过氧化氢传感器 多层自组装 Nation 纳米金 过氧化氢生物传感器 过氧化氢酶 辣根 电极表面 分子自组装 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]