流动注射连续可更新液滴荧光法用于药物制剂中叶酸含量的测定  

Determination of folic acid in pharmaceutical preparations using flow injection continuous renewable drop-based spectrofluorimetry

作　　者：林洁刁 冯锋[1] 陈泽忠[2] 

机构地区：[1]南昌大学化学系,江西南昌330047 [2]山西大同大学化学系,山西大同037009

出　　处：《化学传感器》2005年第3期53-57,共5页Chemical Sensors

基　　金：山西省自然科学基金(No.20051031);山西省重点学科建设基金资助

摘　　要：在酸性条件下,用高碘酸钾作氧化剂,将叶酸氧化成强荧光物质,结合流动注射连续可更新动态液滴荧光法测定叶酸的含量。在优化条件下,体系的荧光强度与叶酸的浓度成线性关系,线性范围为0.02～2.84 mg/L,检测限为0.001 mg/L。该方法灵敏度高,重现性好,操作简便,将其应用于片剂中叶酸含量的测定,结果满意。The content of folic acid was determined using flow injection continuous renewable drop - based spectrofluorimetry based on use of potassium periodate for oxidation of folic acid into strong fluorescence compound in acid medium. Under optimum conditions, the fluorescence intensity is proportional to the concentration of folic acid over the range of 0.02 - 2.84 mg/L, The detection limit is 0. 001 mg/L. The method is sensitive, simple and reproducible. It has been applied to determine folic acid in pharmaceutical preparations with satisfactory results.

关 键 词：流动注射 液滴荧光法 叶酸 高碘酸钾 荧光法测定 更新动态 酸含量 药物制剂 液滴 酸性条件 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置]