小儿慢性病贫血红系祖细胞与肿瘤坏死因子α、白介素6的关系研究  

The relationship between erythroid progenitor cells and tumor necrosis factor α、interleukin-6 in childhood anemia of chronic disease

作　　者：翟晓文[1] 吴玥[1] 陆凤娟[1] 

机构地区：[1]复旦大学附属儿科医院儿科学系,300020

出　　处：《中国小儿血液》2005年第4期149-152,共4页China Child Blood

摘　　要：目的研究检测肿瘤坏死因子α(TNF-α)、IL-6(白介素-6)两种慢性病发生有关的细胞因子水平,以及红系造血祖细胞集落培养生长探讨它们在慢性病贫血(ACD)发病中的作用方法检测病例组为慢性病贫血组(ACD组)患儿,对照组为慢性病无贫血组(NA组)和缺铁性贫血组(IDA组)患儿的血清肿瘤坏死因子α(TNF浕)、白介素-6(IL-6)水平,以及骨髓的红系祖细胞水平(BFU-E、CFU-E)。结果三组BFU-E有差异(F=3.56,P<0.05;三组CFU-E有显著差异(F=8.87,P<0.01)。ACD组与NA组相比,血清TNF-α前者高于后者(t=2.25,P<0.05),两组血清IL-6无差异(t=1.91,P>0.05)。在20例行骨髓培养的慢性病患儿中,血清TNFα与骨髓BFU-E之间无显著相关性(r=-0.37,P>0.05);血清TNF-α与CFU-E之间有负相关(r=-0.57,P<0.05);IL-6与BFU-E、CFU-E无相关性。结论ACD患儿骨髓红系祖细胞BFU-ECFU-E生成受抑制;血清TNF-α、IL-6的升高参与贫血发生;TNFα可能抑制慢性病患儿骨髓CFU-E形成而导致贫血。Objective To study the pathogenesis of childhood anemia of chronic disease（ACD） by measuring two cytokines related with chronic disease and bone marrow erythroid progenitor cells. Methods There were three groups, including case group ACD group, control groups chronic disease without anemia group and iron deficiency anemia group. We measured serum tumor necrosis factor α, interleukin - 6, and bone marrow BFU - E, CFU - E. Result There were different significantly among three groups in BFU - E（ F = 3.56, P 〈 0.05） and CFU- E （F= 8.87,P 〈 0.01 ）. Serum TNF α levels in ACD group were higher than those in NA group（t = 2.25, P 〈 0.05）, and serum IL- 6 levels were no different in two groups（ t = 1.91, P 〉 0.05）. In child with chronic disease, serum TNF α levels were not correlated with bone marrow BFU - E （ r = - 0.37, P 〉 0.05）; serum TNF α levels were negatively correlated with bone marrow CFU- E（r = -0.57, P 〈 0.05）. Serum IL - 6 levels were not correlated with bone marrow BFU - E and CFU - E. Conclusion It suggested that erythroid progenitors bone marrow BFU - E and CFU - E were suppressed. Serum TNF α levels in ACD increased and participated in ACD pregress. TNF a can result in anemia by repressing bone marrow CFU - E.

关 键 词：贫血 慢性病 儿童肿瘤坏死因子 白介素-6 肿瘤坏死因子α(TNF-α) 骨髓红系祖细胞 慢性病贫血 白介素6 肿瘤坏死因子α(TNFα) 血清TNF-Α 血清IL-6 CFU-E 

分 类 号：R725.5[医药卫生—儿科]