针刺胆经对偏头痛大鼠脑干组织G蛋白含量的影响  

Effect of Puncturing Gallbladder Meridian on Content of G Protein in Migraine Model Rat Brain Stem

作　　者：李臻琰[1] 李炜[1] 龚倩[2] 钟广伟[1] 王素娥[1] 文玲波[1] 

机构地区：[1]中南大学湘雅医院,湖南长沙410008 [2]中南大学药学院,湖南长沙410013

出　　处：《湖南中医学院学报》2005年第5期52-54,共3页Journal of Hunan College of Traditional Chinese Medicine

基　　金：湖南省中医药管理局资助项目(200125)

摘　　要：目的研究针刺胆经对实验性偏头痛大鼠脑干组织G蛋白亚基(Gsα和Giα)含量的影响。方法通过皮下注射硝酸甘油(10 mg/kg)建立实验性偏头痛大鼠模型;将动物随机分成正常对照组、生理盐水组、模型对照组和针刺治疗组,运用免疫印迹法(Western blot)检测脑干组织Giα和Gsα的含量。结果皮下注射硝酸甘油4 h后脑干组织Gsα蛋白含量明显升高(P<0.01),Giα蛋白含量明显降低(P<0.01),Gsα/Giα蛋白比值升高;针刺治疗组与模型组比较,脑干组织中Gsα蛋白含量明显降低(P<0.01),Giα蛋白含量明显升高(P<0.01),Gsα/Giα蛋白比值降低。结论偏头痛发作可能与大鼠脑干组织G蛋白信号传导系统功能障碍有关,针刺传导的G蛋白信号通路可能是其防治偏头痛的重要机制之一。Objective To explore the effect of puncturing the gallbladder meridian on the content of G protein alpha subunits in the rat brain stem after nitroglycerin infusion. Methods The model of migraine rats were reproduced in accordance with Knynihar-tassorelli methods by infusing the NO donor nitroglycerin [ glyceryl trintrate （GTN） ]i. h, then the rats were divided randomly into four groups： normal control group, saline control group, model control group and acupuncture treatment group. The content of Gia and Gsa protein in the rat brain stem was analysed by western blot method. Results In the model control group, the content of Gsa protein in the rat brain stem was significantly raised （ P 〈 0.01 ）, the Gia protein was significantly reduced （ P 〈 0.01 ）, and the ration of Gsa/Gia was increased by comparison with the normal control group. In the acupuncture group, Gsa protein in the rat brain stem went down apparently than the model group（ P 〈 0.01 ）, and Gia protein was slightly higher than the model group（ P 〈 0.01 ）, resulting in reducing of GscdGia radons. Conclution The results provide that G-protein-linked signaling system play an important role in the attacking migraine, and the mechanisms of acupuncture on migraine is possibility that the acupuncture mediated the G-protein-linked signal transduction pathway has an important function.

关 键 词：偏头痛 针刺 胆经 G蛋白 大鼠 

分 类 号：R246[医药卫生—针灸推拿学]