射频热毁损治疗肝癌前后血液中AFPmRNA变化及意义  

THE SIGNIFICANCE OF BLOOD AFPmRNA CHANGE IN HEPATOCELLULAR CARCINOMA PATIENTS TREATED WITH RADIOFREQUECY ABLATION

作　　者：杜锡林 马庆久 鲁建国 褚延魁 何显力 臧莉 杨媛 

机构地区：[1]西安第四军医大学第二附属医院,西安710038

出　　处：《肝胆外科杂志》2005年第5期347-349,共3页Journal of Hepatobiliary Surgery

基　　金：陕西省自然科学研究项目(2002C202)

摘　　要：目的探讨肝癌射频热毁损(RFA)治疗前后血液中AFP-mRNA变化及其意义。方法应用RT-PCR检测技术对术前术后48小时,72小时,1周及术后2周肝癌患者周围静脉血液中AFPmRNA,并观察变化。结果183例术前血液中AFPmRNA阴性的肝癌患者中有62例术后72小时转为阳性,但术后1周时复转阴性;而129例术前血液中AFPmRNA阳性的肝癌患者112例术后2w转为阴性;其中17例术后2周仍为阳性,术后证实有病灶残留。结论射频热毁损治疗肝癌可引起血液AFPmRNA一过性升高;血液AFPmRNA可成为判断RFA术后复发或残留的客观性指标之一。Objective To study the significance of blood AFPmRNA in hepatocellular carcinoma （HCC） patients treated with radiofrequency ablation. Methods Using nested RT-PCR,AFPmRNA content in 119 blood sample from the hepatocellular carcinoma patients were determined and the changes observed before radio-frequency abtation ＊ RFA）, 48h, 72h, 1 week and 2 week after RFA. Result In 183 patients with blood AFPmRNA being negative before RFA, positive AFPmRNA was found in 62 cases 72h after RFA and disappeared two week after RFA; 129patients with blood AFPmRNA being positive before RFA,negative AFPmRNA was found in 112 cases two week after RFA; 17 cases was positive two week after RFA. Conclusion RFA lead to change of AFPmRNA in blood ;AFPmRNA was used as a marker of HCC remain and recurrence after RFA.

关 键 词：AFPMRNA 射频热毁损(RFA) 肝细胞癌 RT-PCR 

分 类 号：R735.7[医药卫生—肿瘤]