一氧化氮、过氧亚硝基阴离子对大鼠离体肾缺血再灌注损伤时肾血管反应性的影响  

RENAL VASCULAR RESPONSE TO NITRIC OXIDE AND PEROXYNITRITE IN ISOLATED PERFUSED KIDNEY AFTER RENAL ISCHEMIA REPERFUSION

作　　者：王新良[1] 陈晓玲[2] 谢菲[1] 李洪娟[1] 黄善生[2] 

机构地区：[1]河北医科大学第二医院儿科,河北石家庄050000 [2]河北医科大学基础医学院病理生理学教研室,河北石家庄050017

出　　处：《河北医科大学学报》2005年第6期404-408,共5页Journal of Hebei Medical University

摘　　要：目的探讨一氧化氮、过氧亚硝基阴离子对大鼠离体肾缺血再灌注(ischemia reperfusion,I-R)损伤时肾血管反应性的影响。方法应用离体灌流肾(isolated perfused kidney,IPK)技术观察肾I-R后肾血管对去甲肾上腺素(norepinephrine,NE)和乙酰胆碱(acetylcholine,Ach)反应性的改变;观察外源性给予NO、ONOO-后大鼠IPK肾血管对NE和Ach反应性的改变,以及阻断内源性NO生成对肾I-R大鼠IPK肾血管反应性的影响。结果①I-R后IPK肾血管对去甲肾上腺素(norepinephrine,NE)的缩血管反应性和Ach的舒血管反应性都有明显降低,I-R5h较I-R1h进一步降低(P<0.01)。②给予硝普钠(100μmol/L)后大鼠IPK血管对NE的缩血管反应性和Ach的舒血管反应性都未见明显变化(P>0.05)。而ONOO-(40μmol/L)和vehicle相比则显著降低IPK血管对NE和Ach的反应性(P<0.01)。③氨基胍+I-R1h大鼠IPK肾血管对NE和Ach的反应性与NS+I-R1h大鼠相比无显著差异(P均>0.05),AG+I-R5h组大鼠肾血管对NE和Ach的反应性较NS+I-R5h明显增强(P均<0.05)。L-NNA+I-R 1h和5h大鼠肾血管对Ach的反应性有进一步的降低,而对NE的反应性却增强(P均<0.05)。结论NO大量生成后产生的ONOO-在肾I-R所致的肾血管反应性损伤中具有重要的作用。Objective To investigate renal vascular response to nitric oxide （NO） and peroxynitrite in isolated perfused rat kidney（IPK） after renal ischemia reperfusion（I-R）. Methods IPK was used to examine the effects of renal I-R and nitropru sside（SNP）, ONOO^- on the renal vascular response, and the effects of aminoguanidine on the impaired renal vascular response caused by renal I-R. Results ①Contractile response of renal vascular to norepinephrine（NE） and relaxation responses to acetylcholine（Ach） were both impaired obviously in IPK after renal I-R, and the impaired renal vascular response was more serious in I-Rsh than that in I-R1b（ P 〈0.01）. ②SNP（100μmol/L） did not impact the renal vascular response to NE and Ach（ P 〉0.05） in IPK of health rats, but ONOO^- （40 μmol/L） reduced the renal vascular response to NE and Ach compared with vehicle（ P 〈0.01）. ③There were no difference of the renal vascular response to NE and Ach between aminogu anidine（AG）＋ I-R1b and NS＋ I-R1h （P 〉 0.05 ）, but the renal vascular response were much better in AG＋I-R5h than that in NS＋I-R1h（ P 〈0.05）. The renal vascular response to Ach was further reduced in L-NNA＋I-R than that in NS＋I-R groups, but the renal vascular response to NE was strengthened in L-NNA ＋ I-R group （P 〈 0.05）.Conclusion It is proved that a massive quantity generation of NO and ONOO^- play an important role in renal vascular response damage in IPK rats after renal I-R.

关 键 词：肾 再灌注损伤 一氧化氮 大鼠 

分 类 号：R692.5[医药卫生—泌尿科学]