高效液相色谱法测定血浆中法莫替丁的浓度  

Determination of famotidine in plasma by high performance liquid chromatography

作　　者：杨帆[1] 刘丽京[1] 邓艳萍[1] 

机构地区：[1]北京大学中国药物依赖性研究所,北京100083

出　　处：《黑龙江医药科学》2005年第5期31-32,共2页Heilongjiang Medicine and Pharmacy

摘　　要：目的:用高效液相色谱法测定血浆中法莫替丁浓度.方法:以对乙酰氨基酚为内标,血浆经乙腈提取,HPLC测定.流动相0.01mol·L-1磷酸二氢钾-乙腈(91.5:8.5),流速1.0ml·min-1,C18色谱柱Alltech Apollo (4.6mm×150mm),检测波长266nm.结果:法莫替丁和内标与内源性杂质分离良好;5～160 ng·ml-1浓度范围内线性关系良好;检测限2.5 ng·ml-1;日内精密度RSD为 1.5%～6.7%(n=6),日间精密度RSD为 0.2%～14%(n=6).结论:本法简便、灵敏度较好,可用于法莫替丁血药浓度的测定.Objective： To determinate famotidine in plasma by the high performance liquid chromatography （HPLC）. Methods. Famotidine was extracted from plasma by acetonitrile , then determined by HPLC with an analytical column （4. 6mm × 150mm, 5u） of Alltech Apollo C18. The analytical mobile phase consisted of 0.01 mol · L^-1 KH2PO4-acetonitrile （91.5：8.5）. The flow-rate was 1.0 ml· min^-1, and the wavelength of UV was 266 nm. Results： Famotidine and internal standard can be well separated from endogenous compounds. The calibration curve was linear at the concentrations of 5.0 ～140ng·ml^-1, and the limit of quantitation （LOQ） was 2.5 ng·ml^-1. The RSD value of within-day was 1.5%～6.7%（n=6）, and the RSD value of between-clay was 0.2%～8.5%（n=6）. Conclusion： This method is simple and fast, which can be used for the determination of famotidine in plasma.

关 键 词：高效液相色谱 法莫替丁 血药浓度 

分 类 号：R446.119[医药卫生—诊断学]