单次大容量采集自体外周血干细胞行APBSCT治疗恶性淋巴瘤  

The initial study of a single large volume apheresis session to collect peripheral blood stem cell and then undergo autologous transplantation for lymphoma patients

作　　者：江明[1] 丁凌录[1] 曲建华[1] 钟笛[1] 陈瑢[1] 郝建萍[1] 迪丽娜.阿不来提 王蕾[1] 温丙昭[1] 

机构地区：[1]新疆医科大学第一附属医院血液科新疆维吾尔自治区血液病研究所,新疆乌鲁木齐830054

出　　处：《新疆医科大学学报》2005年第10期968-970,共3页Journal of Xinjiang Medical University

摘　　要：目的:探讨恶性淋巴瘤患者自体外周血干细胞(PBSC)的单次大容量采集方法及其效果。方法:根据7 例恶性淋巴瘤患者动员后外周血的单个核细胞数(MNC)、分离采集过程中收集的干细胞混悬液的混浊程度及病人的状况,采用Cobe-Spectra血细胞分离仪按患者全血容量(TBV)的3个循环单次分离并采集外周血的干祖细胞,计数MNC及CD34+胞数,冻存10-14 d后行自体外周血干细胞移植(APBSCT)术,观察造血重建的情况。结果:7例患者单次大容量采集到的MNC及CD34+平均细胞数分别为7.25×108/kg和5.57×106/kg,采集过程中有1例患者出现较重的肢端及口唇麻木感,余6例均无其他明显不适,采集顺利。行APBSCT术后中性粒细胞≥0.5×109/L及血小板≥20×109/L的平均天数分别为+8.9 d和+8.7 d。结论:部分恶性淋巴瘤患者可单次大容量采集到足够量的干祖细胞,且采集过程是安全的,行APBSCT术后造血重建迅速。Objective： To research the collecting methods of auto-peripheral blood stem cell and it＇s effect. Methods： According to the 7 lymphoma patient＇s MNC of peripheral blood after mobilization, turbidity of the collecting stem cell liquid and the patient＇s status, we used the Cobe-Spectra machine to collect the peripheral blood stem cell in a single apheresis session with 3 total blood volume（TBV） circles. MNC and CD34^＋ cells were calculated, and autologous peripheral blood stem cell transplantation（APBSCT） was underwent after the stem cells cryopreserved 10- 14 days. The hematopoietic rebuilding situation was observed. Results： The 7 patient＇s average MNC and CD34^＋ cells were 7.25× 10^8/kg and 5.57 × 10^6/kg respectively after peripheral blood stem cells harvest in a single large volume apheresis session. The collecting process was favoring. The hematopoietic rebuilt quickly. The average days of neutrophil ≥5× 10^9/L and platelet ≥20× 10^9/L were ＋8.9 and ＋8.7 days respectively after APBSCT. Conclusions： The adequate peripheral blood stem cell of partial lymphoma patients could be collected in a single large volume apheresis session. The collecting process is secure. The hematopoietic rebuild quickly after APBSCT.

关 键 词：单次大容量 干细胞采集 自体外周血干细胞移植 恶性淋巴瘤 

分 类 号：R457.7[医药卫生—治疗学] R733.4[医药卫生—临床医学]