转mtlD基因和BADH基因旱稻苗期耐盐性研究  

The studies on salt-tolerance of transgenic mtlD and BADH upland rice at seedling stage

作　　者：张丽[1] 张洪亮[1] 李自超[1] 

机构地区：[1]中国农业大学农业部作物基因组学与遗传改良重点实验室北京市作物遗传改良实验室,北京100094

出　　处：《西南农业学报》2005年第5期621-624,共4页Southwest China Journal of Agricultural Sciences

基　　金：国家转基因植物研究与产业化开发专项(JY03-13-10)资助

摘　　要：对同时含有mtlD和BADH基因的旱稻,只含mtlD基因或只含BADH基因的旱稻,非转基因旱稻三者在盐胁迫下的表型,生长速率,相对电导率以及K+/Na+含量的比较,发现它们的耐盐性的关系是:同时含有mtlD和BADH基因旱稻>只含mtlD基因或只含BADH旱稻>非转基因旱稻,从而推断出mtlD基因和BADH基因在旱稻的耐盐性方面具有协同(或累加)效应。通过试验比较,还发现只含有mtlD基因和只含有BADH基因的旱稻的耐盐性没有明显差别,从而推断出mtlD基因和BADH基因对植物的耐盐性的贡献差异不显著。此外,已经得到的转基因材料也可以继续作为进一步转化的材料,这为我们进行多基因转化改良植物提供了一条新的途径。The salt-tolerant ability test including the phenotype, relative electric conductivity,and the K^＋ / Na^＋ content indicates that the plants, which had been transformed two genes, had the highest salt-tolerant ability. The plant only with mtlD gene or only with BADH gene had secondly ability, and the nontrarksgenic plants was the third. So we concluded that the two genes （mtlD and BADH） have additive effects of salt-tolerance. We also conclude that there is no difference between the effects of mtlD gene and BADH gene in salt - tolerance. Besides, the transgenlc material with one exogenous gene could be used as a new material for further transformation; this is a new road to improve a plant by polygenes transformation.

关 键 词：早稻 1-磷酸甘露醇脱氢酶(mtlD) 甜菜碱醛脱氢酶(BADH) 耐盐性 

分 类 号：S332.6[农业科学—作物遗传育种]