一个新的编码大豆DREB转录因子基因的克隆及鉴定  

Cloning and identification of a new gene encoding DREB transcription factors from Glycine max L.

作　　者：王巧燕[1] 陈明[1] 邱志刚[1] 程宪国[2] 徐兆师[1] 李连城[1] 马有志[1] 

机构地区：[1]中国农业科学院作物科学研究所农作物基因资源与基因改良国家重大科学工程农业部作物遗传育种重点开放实验室 [2]中国农业科学院资源与农业区划研究所,北京100081

出　　处：《西南农业学报》2005年第5期625-628,共4页Southwest China Journal of Agricultural Sciences

基　　金：国家"863"项目(2002AA224081);国家植物转基因研究与产业化项目(JY03-A-18)

摘　　要：DREB转录因子是一类可以调控多个与干旱、高盐及低温耐性有关的功能基因表达的转录因子家族。从大豆耐盐品种铁丰8号中克隆了一个新的DREB基因GmDREB5。该基因编码309个氨基酸,具有典型的AP2/EREBP保守结构域,属于AP2/EREBP类转录因子中的DREB亚族。同源性比较分析表明,GmDREB5基因与Genbank登录的DREB基因同源性不高,属于新基因。酵母转录激活实验证明,该基因可以与DRE顺式作用元件特异结合,并具有转录激活活性;同时采用CaMV35S启动子驱动,构建了植物表达载体pBI35S-GmDREB5,并通过冻融法将重组质粒导入根癌农杆菌EHA105中,再利用叶盘转化法将重组质粒导入烟草品种W38中,获得转基因烟草植株30株。DREB transcription factors are kinds of controlling the expression of several target function genes involved in plant tolerance to drought,high-salt and cold stress. A novel full length gene, GmDREB5, was successfully cloned from Glycine max （L.） Merr in this study. Further study indicated that the gene coded 309 amino acids, contained AP2/EREBP domain and belonged to DREB subfamily. By phylogenetic analysis of GmDREB5 and other GmDREBs in sequence, the gene was proved to be a new DREB. The gene was demonstrated had transcription activation function according to yeast transcription activation experiment. A plant transformation vector pBI35SC-mDREB5 was constructed, on which GrnDREB5 gene was controlle by CaMV35s promoter, and the recombination plasmid was transferred into tobacco mediated with agrobacterium via leaf-disc method, and 30 transgenic plants were obtained in total.The functional identification of the transgenic individuals for tolerance to drought, high-salt and cold stress is conducting smoothly.

关 键 词：DREB转录因子 转录激活CaMV35S启动子 植物表达载体 

分 类 号：S336[农业科学—作物遗传育种]