Effects of propyl gallate on interaction between TNF-α and sTNFR-I using an affinity biosensor  

作　　者：Jing LI Jia-dong HUANG Bao-yan WU Qiang CHEN 

机构地区：[1]Laboratory of Biosensors, College of Life Science, Nankai University, Tianjin 300071, China

出　　处：《Acta Pharmacologica Sinica》2005年第10期1212-1216,共5页中国药理学报（英文版）

基　　金：Project supported by the National Natural Science Foundation of China (№ 90209054)

摘　　要：Aim： To study the effects of propyl gallate on the interaction of tumor necrosis factor-α （TNF-α） with its soluble receptor, sTNFR-I. Methods： Interactions between TNF-α and sTNFR-I were analyzed using an IAsys biosensor, sTNFR-I was immobilized on the carboxymethyl dextran （CMD） surface of the IAsys biosensor cuvettes, and TNF-α preincubated with different concentrations of propyl gallate was added to the cuvettes. The resonant angle shift caused by the binding between TNF-α and sTNFR-I was then recorded. Results： sTNFR-I was immobi- lized on the CMD surface at a density of 2.76 ng/mm^2. TNF-α then bound the immobilized sTNFR-I specifically, and propyl gallate was able to enhance the binding between TNF-α and sTNFR-I in a dose-dependent manner. Conclusion： The binding between TNF-α and sTNFR-I is one of the targets that propyl gallate can act on in vivo. The IAsys biosensor offers a new clue as to the study on the mechanisms of action of propyl gallate.Aim： To study the effects of propyl gallate on the interaction of tumor necrosis factor-α （TNF-α） with its soluble receptor, sTNFR-I. Methods： Interactions between TNF-α and sTNFR-I were analyzed using an IAsys biosensor, sTNFR-I was immobilized on the carboxymethyl dextran （CMD） surface of the IAsys biosensor cuvettes, and TNF-α preincubated with different concentrations of propyl gallate was added to the cuvettes. The resonant angle shift caused by the binding between TNF-α and sTNFR-I was then recorded. Results： sTNFR-I was immobi- lized on the CMD surface at a density of 2.76 ng/mm^2. TNF-α then bound the immobilized sTNFR-I specifically, and propyl gallate was able to enhance the binding between TNF-α and sTNFR-I in a dose-dependent manner. Conclusion： The binding between TNF-α and sTNFR-I is one of the targets that propyl gallate can act on in vivo. The IAsys biosensor offers a new clue as to the study on the mechanisms of action of propyl gallate.

关 键 词：propyl gallate tumor necrosis factor-α soluble tumor necrosis factor receptor affinity biosensor 

分 类 号：R945[医药卫生—微生物与生化药学]