Assessing phylogenetic relationships of Lycium samples using RAPD and entropy theory  

作　　者：Xiao-lin YIN Kai-tai FANG Yi-zeng LIANG Ricky NS WONG Amber WY HA 

机构地区：[1]School of Mathematics and Statistics, Northeast Normal University, Changchun 130024, China [2]Department of Mathematics, Hong Kong Baptist University, Hong Kong, China [3]College of Chemistry and Chemical Engineering, Central South University, Changsha 410083,China [4]Department of Biology, Hong Kong Baptist University, Hong Kong, China

出　　处：《Acta Pharmacologica Sinica》2005年第10期1217-1224,共8页中国药理学报（英文版）

基　　金：Project supported by the National Natural Science Foundation of China (№ 10371015 and 10329102);a grant from the Hong Kong Baptist University (№ FRG/02-03/II-62)

摘　　要：Aim： To evaluate the phylogenetic relationships among related species of Lycium samples. Methods： Random amplified polymorphic DNA （RAPD） fingerprinting and lab-on-a-chip electrophoresis techniques were used to analyze the characteristics of Lycium species. Seven species and 3 varieties of Lycium were studied. Based on RAPD fingerprint data obtained from 11 primers, we proposed a new index, called dispersivity, using entropy theory and projection methods to depict the diversity of the DNA fingerprints. Results： Using the proposed dispersivity, primers were sorted and the dendrograms of the 7 species and 3 varieties of Lycium were constructed synthetically by merging primer information. Conclusion： Phylogenetic relationships among Lycium samples were constructed synthetically based on RAPD fingerprint data generated from 11 primers.Aim： To evaluate the phylogenetic relationships among related species of Lycium samples. Methods： Random amplified polymorphic DNA （RAPD） fingerprinting and lab-on-a-chip electrophoresis techniques were used to analyze the characteristics of Lycium species. Seven species and 3 varieties of Lycium were studied. Based on RAPD fingerprint data obtained from 11 primers, we proposed a new index, called dispersivity, using entropy theory and projection methods to depict the diversity of the DNA fingerprints. Results： Using the proposed dispersivity, primers were sorted and the dendrograms of the 7 species and 3 varieties of Lycium were constructed synthetically by merging primer information. Conclusion： Phylogenetic relationships among Lycium samples were constructed synthetically based on RAPD fingerprint data generated from 11 primers.

关 键 词：DNA fingerprinting RAPD technique labon-a-chip entropy UPGMA 

分 类 号：Q983.61[生物学—人类学]