Effect of daidzin,genistin,and glycitin on osteogenic and adipogenic differentiation of bone marrow stromal cells and adipocytic transdifferentiation of osteoblasts  

作　　者：Xiang-hui LI Jin-chao ZHANG Sen-fang SUI Meng-su YANG 

机构地区：[1]State Key Laboratory of Biomembrane, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China [2]Department of Chemistry, College of Chemistry and Environmental Science, Hebei University, Baoding 071002, China [3]Department of Biology and Chemistry City University of Hong Kong,Hong Kong, China

出　　处：《Acta Pharmacologica Sinica》2005年第9期1081-1086,共6页中国药理学报（英文版）

摘　　要：Aim： To examine the effect of daidzin, genistin, and glycitin on the osteogenic and adipogenic differentiation of bone marrow stromal cells （MSC） and the adipogenic transdifferentiation of osteoblasts. Methods： MTT test, alkaline phosphatase （ALP） activity measurement, Oil Red O stain and measurement were employed. Results： Daidzin, genistin, and glycitin 1×10^-8, 5×10^-7, 1×10^-6, 5×10^-6, and 1×10^-5 mol/L all promoted the proliferation of primary mouse bone MSC and osteoblasts. Daidzin 5×10^-7 mol/L and genistin 1×10^-6 mol/L promoted the osteogenesis of MSC. Genistin 1×10^-8, 5×10^-7, 1×10^-6, 5×10^-6, and 1×10^-5 mol/L and glycitin 1×10^-8, 1×10^-6, and 1×10^-5 mol/L inhibited the adipogenesis of MSC. Daidzin, genistin, and glycitin 1×10^-8, 5×10^-7, 1 ×10^-6, 5×10^-6, and 1 ×10^-5 mol/L all inhibited the adipocytic transdifferentiation of osteoblasts. Conclusions： Daidzin, genistin, and glycitin may modulate differentiation of MSC to cause a lineage shift toward the osteoblast and away from the adipocytes, and could inhibit adipocytic transdifferentiation of osteoblasts. They could also be helpful in preventing the development of osteonecrosis.Aim： To examine the effect of daidzin, genistin, and glycitin on the osteogenic and adipogenic differentiation of bone marrow stromal cells （MSC） and the adipogenic transdifferentiation of osteoblasts. Methods： MTT test, alkaline phosphatase （ALP） activity measurement, Oil Red O stain and measurement were employed. Results： Daidzin, genistin, and glycitin 1×10^-8, 5×10^-7, 1×10^-6, 5×10^-6, and 1×10^-5 mol/L all promoted the proliferation of primary mouse bone MSC and osteoblasts. Daidzin 5×10^-7 mol/L and genistin 1×10^-6 mol/L promoted the osteogenesis of MSC. Genistin 1×10^-8, 5×10^-7, 1×10^-6, 5×10^-6, and 1×10^-5 mol/L and glycitin 1×10^-8, 1×10^-6, and 1×10^-5 mol/L inhibited the adipogenesis of MSC. Daidzin, genistin, and glycitin 1×10^-8, 5×10^-7, 1 ×10^-6, 5×10^-6, and 1 ×10^-5 mol/L all inhibited the adipocytic transdifferentiation of osteoblasts. Conclusions： Daidzin, genistin, and glycitin may modulate differentiation of MSC to cause a lineage shift toward the osteoblast and away from the adipocytes, and could inhibit adipocytic transdifferentiation of osteoblasts. They could also be helpful in preventing the development of osteonecrosis.

关 键 词：DAIDZIN GENISTIN glycitin bone marrow stromal cells osteogenic differentiationadipogenic differentiation osteoblastsadipocytic transdifferentiation 

分 类 号：R68[医药卫生—骨科学]