Association of polymorphisms in low-density lipoprotein receptor-related protein 5 gene with bone mineral density in postmenopausal Chinese women  

作　　者：Zhen-lin ZHANG Yue-juan QIN Jin-wei HE Qi-ren HUANG Miao LI Yun-qiu HU Yu-juan LIU 

机构地区：[1]Center for Preventing and Treating Osteoporosis, Osteoporosis Research Unit, the Sixth People's Hospital, Shanghai Jiaotong University, Shanghai 200233, China

出　　处：《Acta Pharmacologica Sinica》2005年第9期1111-1116,共6页中国药理学报（英文版）

基　　金：the grant from the Natural Science Foundation of Shanghai(No 03ZR 14056).

摘　　要：Aim： To investigate the possible association of Q89R, N740N and A 1330V polymorphisms in low-density lipoprotein receptor-related protein 5 （LRP5） gene with bone mineral density （BMD） in postmenopausal Chinese women. Methods： Q89R, N740N and A1330V genotypes were determined by polymerase chain reactionrestriction fragment length polymorphism （PCR-RFLP） in 647 unrelated healthy postmenopausal Han Chinese women aged 43-76 years in Shanghai. BMD at lumbar spine 1-4 and the left proximal femur including the femoral neck, trochanter and Ward＇s triangle were measured by dual-energy X-ray absorptionmetry in all subjects. Results： The distribution of the Q89R, N740N and A 1330V genotypes in this population was as follows： QQ 80.5%, QR 18.7%, and RR 0.8%; TT 66.9%, TC 31.1%, and CC 2.0%; AA 68.0%, AV 29.7%, and VV 2.3%. The frequencies of the Q89R, N740N and A 1330V genotypes and alleles did not deviate from the Hardy- Weinberg equilibrium. We found that the Q89R and AI330V polymorphisms were in linkage disequilibrium in our population （X^2=13.50, P〈0.01 ）. Both before and after adjusting for age, years since menopause, height, and weight, the Q89R or N740N genotypes were significantly associated with BMD at the femoral neck （P〈0.05）. Subjects with the Q89R QQ genotype or the N740N TT genotype had a significantly higher BMD at the femoral neck, compared with those with the QR/RR or TC/CC genotypes, respectively. No significant association was found between A1330V polymorphism and BMD at any site. Conclusion： Our findings suggest that the LRP5 gene is a candidate for the genetic determination of BMD in postmenopausal Chinese women.Aim： To investigate the possible association of Q89R, N740N and A 1330V polymorphisms in low-density lipoprotein receptor-related protein 5 （LRP5） gene with bone mineral density （BMD） in postmenopausal Chinese women. Methods： Q89R, N740N and A1330V genotypes were determined by polymerase chain reactionrestriction fragment length polymorphism （PCR-RFLP） in 647 unrelated healthy postmenopausal Han Chinese women aged 43-76 years in Shanghai. BMD at lumbar spine 1-4 and the left proximal femur including the femoral neck, trochanter and Ward＇s triangle were measured by dual-energy X-ray absorptionmetry in all subjects. Results： The distribution of the Q89R, N740N and A 1330V genotypes in this population was as follows： QQ 80.5%, QR 18.7%, and RR 0.8%; TT 66.9%, TC 31.1%, and CC 2.0%; AA 68.0%, AV 29.7%, and VV 2.3%. The frequencies of the Q89R, N740N and A 1330V genotypes and alleles did not deviate from the Hardy- Weinberg equilibrium. We found that the Q89R and AI330V polymorphisms were in linkage disequilibrium in our population （X^2=13.50, P〈0.01 ）. Both before and after adjusting for age, years since menopause, height, and weight, the Q89R or N740N genotypes were significantly associated with BMD at the femoral neck （P〈0.05）. Subjects with the Q89R QQ genotype or the N740N TT genotype had a significantly higher BMD at the femoral neck, compared with those with the QR/RR or TC/CC genotypes, respectively. No significant association was found between A1330V polymorphism and BMD at any site. Conclusion： Our findings suggest that the LRP5 gene is a candidate for the genetic determination of BMD in postmenopausal Chinese women.

关 键 词：bone density low-density lipoprotein receptorrelated protein 5 POLYMORPHISM 

分 类 号：R711.51[医药卫生—妇产科学]