抗水貂肠炎细小病毒单克隆抗体的建立  

Production of Monoclonal Antibodies Against Mink En-teritis Parvovirus

作　　者：王克坚[1] 吴威[1] 聂金珍[1] 程世鹏[1] 崔俊青 郭立军[1] 

机构地区：[1]中国农业科学院特产研究所,卫生部长春生物制品研究所

出　　处：《中国兽医学报》1996年第1期48-49,共2页Chinese Journal of Veterinary Science

基　　金：吉林省青年科学基金

摘　　要：以我国分离的水貂肠炎细小病毒（ＭＥＶ）为抗原免疫ＢＡＬＢ／ｃ小鼠获得的脾细胞与ＮＳ－１骨髓瘤细胞融合，筛选出８株分泌抗ＭＥＶ的单克隆抗体杂交瘤细胞。经鉴定，８株杂交瘤细胞都属于Ｂ型抗ＭＥＶ单克隆抗体。BALB/c mice were immunized with cell culture preparation of the minkenteritis virus(MEV),and their splenocyte;were fused with NS-1 myeloma cells. 8 hy-bridoma cell lines secreting specific monoclonal antibodies against mink enteritis par-vovirus were produced after cloning by limiting dilution three times.The eight hybrido-ma cell lines were all identified as to be type B McAbs by reacting with all serotypes ofA,B,C MEV respectively using ELISA.Therefore,this is the first production of the hy-bridoma cells in China that secret type B monoclonal antibody against mink enteritis par-vovirus.The availability of McAbs to MEV may facilitate development of an applicablevaccine for immunization to MEV in China。

关 键 词：水貂 肠炎 细小病毒 单克隆抗体 

分 类 号：S852.6[农业科学—基础兽医学] S858.951[农业科学—兽医学]