TNF增强多聚不饱和脂肪酸诱导中性白细胞超氧基释放  

Polyunsaturated fatty acids induced superoxide release from human neutrophils enhanced by tumor necrosis factor α

作　　者：李永琴[1] 

机构地区：[1]贵阳医学院,550004

出　　处：《中华微生物学和免疫学杂志》1996年第1期49-53,共5页Chinese Journal of Microbiology and Immunology

摘　　要：超氧基是吞噬细胞呼吸爆发系列酶反应的主要代谢产物，它对抗感染、抗肿瘤、增强机体防御能力有重要意义。用Ｈｙｐａｑｕｌｅ－ｆｉｃｏｌｌ梯度离心法从健康人外周血分离的中性白细胞（ＰＭＮ）与肿瘤坏死因子（ＴＮＦα）和多聚不饱和脂肪酸（ＰＵＦＡ）分别或先后温育，用化学发光法（Ｃｈｅｍｉｌｕｍｉｎｅｓｃｅｎｃｅ，ＣＬ）和超氧基歧化酶（ＳＯＤ）抑制高铁细胞色素Ｃ还原法测定被激发细胞的超氧基释放水平，两种测定法获得一致的结果。研究结果表明，包括花生四烯酸（ＡＡ）、二十碳五烯酸（ＥＰＡ）、二十二碳六烯酸（ＤＨＡ）等ＰＵＦＡ均能明显加强ＰＭＮ的超氧基释放。经ＴＮＦα预处理的细胞，超氧基释放量明显增加。超氧基释放水平与ＰＵＦＡ和ＴＮＦα使用浓度呈正相关。Using hypaque-ficoll differential density centrifugation, we prepared polymorpho nuclear nutrophil leucocytes(PMNs)from peripheral blood of healthy adult donors. The cells were pretreated with tumor necrosis factor α(TNFα) of/then treated with polyunsaturated fatty acids (PUFA) . Comparing the two methods of lucigenin-dependent chemiluminescence and superoxide production determined by superoxide dismutase(SOD) inhibiting reduction of cytcthrom C , we have obtained similar results. PUFA including arachidonic acid (AA,20:4),ecosapentaenoic acid ( EPA , 20 : 5 ) , docosahexaenoic acid (DHA , 22: 6 ) induced superoxide release of neutrophils. The cells pretreated with TNF were more sensitive to PUFA for enhanced superoxide generation significantly. Also , we found that the .superoxide levels increased were dose dependent of PUFA and TNFα

关 键 词：多形核 中性白细胞 肿瘤坏死因子 PUFA 

分 类 号：R392.12[医药卫生—免疫学]