大鼠坐骨神经去细胞基膜管异体移植的比较研究  

Experimental study on repairing sciatic nerve defects by two kinds of acellular basal lamina grafts

作　　者：丁海[1] 周建生[1] 胡汝麒[1] 

机构地区：[1]蚌埠医学院附属医院骨科,安徽蚌埠233004

出　　处：《蚌埠医学院学报》2005年第6期479-482,F0004,共5页Journal of Bengbu Medical College

基　　金：安徽省科技厅"十五"科技攻关项目(G322011)

摘　　要：目的:探讨化学萃取法(CEN)、冻融法(FTN)制备的异种鼠坐骨神经去细胞基膜管移植术后8、13、23天神经再生轴突距离、Schwann细胞迁入、再血管化及炎症反应异同。方法:将36只雌性Wistar鼠随机配对,分为CEN组与FTN组,每组18只,分别桥接移植两种方法制备的SpragueDawley鼠坐骨神经去细胞基膜管。观察术后8、13、23天移植物物理性状及组织形态学变化。结果:术后8、13、23天,神经轴突再生距离、近和远端Schwann细胞数、再血管化及炎症反应差异有显著性(<0.05),CEN组优于FTN组。结论:化学萃取法制备的异种周围神经去细胞基膜管较冻融法能更快、更好地修复周围神经缺损,亦应有更大的临床实用价值。Objective： To explore the difference in axon regeneration distance, Sehwann cells, visualization of blood vessels and macrophages of two kinds of allografted acellular basal lamina tubes pretreated respectively by chemical extraction procedure and freeze-thawed procedure.Methods：Thirty six Wistar rats were divided randomly into two groups, chemical extracted nerve（CEN） group and freeze-thawed nerve（FTN） group, which were aUografted by two kinds of acellular basal lamina tubes of Sprague-Dawley rats＇ sciatic nerves.At day 8, 13 and 23 after the operation, the grafts, together with a proximal and distal nerve segment, were removed and were observed by histochemistry and immunohistochemistry to reveal the morphological difference. Results： CEN was better than FIN at supporting the outgrowth of axon, migration of Schwann cells, visualization of blood vessels and maerophages. Conclusions：The chemical extraction procedure can offer a better solution to the repair of a gap in the continuity of the peripheral nerve.

关 键 词：移植 异体 坐骨神经 去细胞基膜管 化学萃取 冻融 SCHWANN细胞 大鼠 

分 类 号：R617[医药卫生—外科学]