妊娠期高血压疾病患者血小板活化检测的临床意义  

Clinical significance of detecting the activation of blood platelet in patients with hypertension disorder in pregnancy by flow cytometry

作　　者：靳丽杰[1] 吕合作[2] 杜丹丽[1] 席玉玲[1] 赵发英[1] 李柏青[2] 

机构地区：[1]蚌埠医学院附属医院妇产科,安徽蚌埠233004 [2]蚌埠医学院免疫学教研室,安徽蚌埠233003

出　　处：《蚌埠医学院学报》2005年第6期502-504,共3页Journal of Bengbu Medical College

摘　　要：目的:探讨妊娠期高血压疾病患者血小板活化情况及临床意义。方法:采用全血法流式细胞术(flowcytometry,FCM)检测妊娠期高血压疾病患者血小板膜表面活化纤维蛋白原受体(FIBR)的表达,同时用发色底物法检测实验对象的凝血四项,并与对照组检测结果比较。结果:妊娠期高血压疾病患者血小板活化的强度和比例均明显高于正常妊娠组和非妊娠组妇女(P<0.01),三者的凝血酶原时间和活化部分凝血酶原时间差异均有显著性(P<0.01),而凝血时间和纤维蛋白原差异均无显著性(P>0.05)。结论:同常规凝血四项检测相比,全血法FCM检测血小板膜表面活化FIBR可灵敏地反映患者血小板活化状态,判断妊娠期高血压疾病病情,为预测和诊断子疒间前期以及是否有DIC倾向提供一项早期指标。Objective：To explore the clinical significance the activation state of blood platelet in patients with hypertension disorder in pregnancy. Methods： The expression of activated fibrinogen receptor on the surface of blood platelet in patients with hypertension disorder in pregnancy was determined in whole blood by flow cytometry（FGM） ,and four parameters of blood coagulation were tested by chromogenic assays. Results：The intensity and the ratio of activated blood platelet in pregnant women with hypertension disorder were significantly higher than these in normal gravida and non-pregnant women （P 〈 0.01）. There was significant difference in prothrombin time（PT） and activated partial thromboplatin time（APTT） between pregnant women with hypertension disorder,normal gravida and non-pregnant women, but no significant difference of coagulation time and fibrinogen between them was found. Conclusions： Comparing with assay of four parameters of blood coagulation, detection of activated fibrinogen receptor on surface of blood platelet in whole blood by FCM can reflect the activation state of blood platelet and evaluate the state of the disease in pregnant women with hypertension disorders. It can serve as an early indicator to predict and diagnose preeclampsi of the disease and tendency of disseminated intravascular coagulation.

关 键 词：妊娠并发症 妊娠期高血压疾病 血小板活化 流式细胞术 纤维蛋白原受体 

分 类 号：R714.253[医药卫生—妇产科学]