Interaction between poly(amidoamine) dendrimers and DNA studied by spectroscopic methods  

作　　者：WANG Yanming SONG Yu KONG Deling YU Yaoting 

机构地区：[1]Bioactive Material Research Laboratory, Nankai University, Tianjin300071, China

出　　处：《Chinese Science Bulletin》2005年第19期2161-2165,共5页

摘　　要：The interaction between amino-terminated, and ethylenediamine core poly(amidoamine) (PAMAM) den-drimers and herring sperm DNA was investigated by various spectroscopic methods including UV spectroscopy, fluores-cence spectroscopy, microscopic FTIR- and circular dichro-ism (CD-) spectroscopy. Ethidium bromide (EB) is used as a nucleic acid probe for this study. Experimental results show that PAMAM dendrimers can form stable complexes with DNA and the dendrimers bind to DNA sufficiently strong which cannot be displaced by EB, and we also found that the formation of the complexes can cause the conformation change of the DNA secondary structure. According to the Scatchard analysis, the association constant of PAMAM to DNA is calculated to be 2.53 × 104 mol/L?1.The interaction between amino-terminated, and ethylenedtamine core poly（amtdoamine） （PAMAM） dendrimers and herring sperm DNA was investigated by various spectroscopic methods including UV spectroscopy, fluorescence spectroscopy, microscopic FTIR- and circular dichroism （CD-） spectroscopy. Ethidium bromide （EB） is used as a nucleic acid probe for this study. Experimental results show that PAMAM dendrimers can form stable complexes with DNA and the dendrimers bind to DNA sufficiently strong which cannot be displaced by EB, and we also found that the formation of the complexes can cause the conformation change of the DNA secondary structure. According to the Scatchard analysis, the association constant of PAMAM to DNA is calculated to be 2.53×10^4 mol/L^-1.

关 键 词：聚乙烯 分光镜 分子结构 DNA 荧光性 

分 类 号：Q523[生物学—生物化学]