Distribution of a kinesin-related protein on Golgi apparatus of tobacco pollen tubes  

作　　者：WEI Liqin LIU Bo LI Yan 

机构地区：[1]State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100094, China [2]Section of Plant Biology, University of California-Davis, Davis, CA 95616-8537, USA

出　　处：《Chinese Science Bulletin》2005年第19期2175-2181,共7页

基　　金：This work was supported by the National Natural Science Foundation of China fGrant Nos．30470109＆30421002)．

摘　　要：Previous study has shown that the internal mo-tor kinesin AtKinesin-13A is associated with Golgi stacks in various Arabidopsis cells. With degenerate primers, a seg-ment of 2067 bp was cloned from tobacco by RT-PCR. The polypeptide coded by the segment has 77.33% similarities with the corresponding part of AtKinesin-13A from Arabi-dopsis; and antibody of AtKinesin-13A recognized specifi-cally the expressed products of the segment in bacteria. Us-ing the anti-AtKinesin-13A antibodies, a 120 kD polypeptide was detected from protein extracts of various organs from Nicotiana tabacum, indicating that a homologue of AtKine-sin-13A protein, NtKinesin-13A, is expressed in Nicotiana tabacum. Immunofluorescence staining showed that the to-bacco NtKinesin-13A protein appeared in particles in pollen tubes by confocal microscopy. Immunofluorescence double labeling showed that the NtKinesin-13A protein particles partially co-distributed with 58K protein, indicating that NtKinesin-13A is located on Golgi apparatus. Immuno-gold labeling and electron microscopy observation revealed that the NtKinesin-13A was associated with Golgi-associated vesi-cles membranes in tobacco pollen tubes. These results sug-gest that the NtKinesin-13A may probably play an important role in controlling Golgi stacks, and/or in transporting Golgi- derived vesicles in pollen tubes.Previous study has shown that the internal motor kinesin AtKinesin-13A is associated with Golgi stacks in various Arabidopsis cells. With degenerate primers, a segment of 2067 bp was cloned from tobacco by RT-PCR. The polypeptide coded by the segment has 77.33% similarities with the corresponding part of AtKinesin-13A from Arabidopsis; and antibody of AtKinesin-13A recognized specifi. cally the expressed products of the segment in bacteria. Using the anti-AtKinesin-13A antibodies, a 120 kD polypeptide was detected from protein extracts of various organs from Nicotiana tabacum, indicating that a homologue of AtKine- sin-13A protein, NtKinesin-13A, is expressed in Nicotlana tabacum. Immunofluorescence staining showed that the tobacco NtKinesin-13A protein appeared in particles in pollen tubes by confocal microscopy. Immunofluorescence double labeling showed that the NtKinesin-13A protein particles partially co-distributed with 58K protein, indicating that NtKinesin-13A is located on Golgi apparatus, lmmuno-gold labeling and electron microscopy observation revealed that the NtKinesin-13A was associated with Golgi-associated vesicles membranes in tobacco pollen tubes. These results suggest that the NtKinesin-13A may probably play an important role in controlling Golgi stacks, and/or in transporting Golgiderived vesicles in pollen tubes.

关 键 词：Golgi器械 花粉管 蛋白质 烟草 微生物 

分 类 号：Q946.1[生物学—植物学]