7株绿僵菌防治德国小蠊的毒力筛选  

THE VIRULENCE SELECTION AMONG 7 METASHIZIUM STRAINS AGAINST THE GERMAN COCKROACHES

作　　者：任向辉[1] 焦名琼[1] 张艳[1] 何国锋[1] 

机构地区：[1]中山大学昆虫学研究所生物防治国家重点实验室,510275

出　　处：《昆虫天敌》2005年第2期57-62,共6页Natural Enemies of Insects

摘　　要：本文用7个绿僵菌(Metarhizium)菌株对德国小蠊Blattella germanica(Linnaeus)进行注射生测,筛选出了一个致病菌株进行五代复壮选育.该株绿僵菌Metarhizium anisopli-ae(Metsch)Sorokin复壮前对雌雄成虫注射生测LD50依次为13.2与8.9天;复壮后对雌雄成虫背部点滴生测12天的LD50值均为1.3×105孢子/虫.通过CP包埋剂制作石蜡切片对僵虫体内菌丝核和体表孢子堆进行了观察.The virulence of 7 Metarhizium strains against the German cockroaches Blattella germanica （Linnaeus） was selected by injection method, and a pathogenic strain Metarhizium anisopliae （Metsch） Sorokin was passed through five times rejuvenation. The LT50 values of the bioassay by injection method were 13.2d （female） and 8.9d （male） before the rejuvenation; the LD50 （12 day） was 1.3 × 10^5 spores per cockroach after the rejuvenation. Using CP embedding tissue section technique, the microstructure of the muscardine cadavers＇ soft and mycelial mass was observed.

关 键 词：生物测定 绿僵菌 德国小蠊 僵虫 

分 类 号：S476.12[农业科学—农业昆虫与害虫防治]