Isolation, cloning and sequencing of AFLP markers related to disease-resistance traits in Fenneropenaeus chinensis  

作　　者：岳志芹 王伟继 孔杰 戴继勋 

机构地区：[1]Laboratory for Sustainable Utilization of Marine Fisheries Resources, Yellow Sea Fisheries Research Institute,Qingdao 266071,China [2]College of Marine Life Science, Ocean University of China, Qingdao 266003, China [3]Food Laboratory of Qingdao Entry-Exit Inspection and Quarantine Bureau, Qingdao 266001, China [4]College of Marine Life Science, Ocean University of China,Qingdao 266003, China

出　　处：《Chinese Journal of Oceanology and Limnology》2005年第4期442-447,共6页中国海洋湖沼学报（英文版）

基　　金：This research was supported by special funds from the National KeyBasic Research Program (G1999012007) and the National High-TechResearch and Development Program of China (863 Program,2001AA620105)

摘　　要：Amplified fragment length polymorphisms (AFLP) technique was used to analyze the fingerprint- ing of four successive generations of Fenneropenaeus chinensis to reveal their disease-resistance traits. Some loci showed quite different genetic frequencies due to artificial selection, which implied that these fragments were putative markers related to the disease-resistance trait. We developed a simple and effective method to fur- ther characterize these AFLP fragments. Specific AFLP bands were cut directly from polyacrylamide gels, re-amplified, cloned and sequenced. Eight putative genetic markers were sequenced and their sizes ranged from 63 to 209 bp. The sequences were submitted to dbGSS (database of Genome Sequence Survey); and the BLAST analysis showed low similarity to the function genes, indicating these markers were tightly linked to a dis- ease-resistance trait but were not functional genes.Amplified fragment length polymorphisms （AFLP） technique was used to analyze the fingerprinting of four successive generations of Fenneropenaeus chinensis to reveal their disease-resistance traits. Some loci showed quite different genetic frequencies due to artificial selection, which implied that these fragments were putative markers related to the disease-resistance trait. We developed a simple and effective method to further characterize these AFLP fragments. Specific AFLP bands were cut directly from polyacrylamide gels, re-amplified, cloned and sequenced. Eight putative genetic markers were sequenced and their sizes ranged from 63 to 209 bp. The sequences were submitted to dbGSS （database of Genome Sequence Survey）; and the BLAST analysis showed low similarity to the function genes, indicating these markers were tightly linked to a disease-resistance trait but were not functional genes.

关 键 词：AFLP Fenneropenaeus chinensis disease-resistance trait markers CLONE sequence 

分 类 号：S945.1[农业科学—水产养殖]