海南岛番木瓜和扶桑上粉虱传双生病毒的检测及序列分析  

Detection and Partial Sequence Analysis of Whitefly-transmitted Geminivirus in Carica papaya and Hibiscus rosa-sinensis in Hainan

作　　者：车海彦[1] 罗大全[1] 杨旭光[1] 符瑞益[1] 叶莎冰[1] 

机构地区：[1]中国热带农业科学院环境与植物保护研究所,海南儋州571737

出　　处：《中国病毒学》2005年第5期543-545,共3页Virologica Sinica

基　　金：中国热带农业科学院科技基金(Rky0303)

摘　　要：Three virus-infected Carica papaya samples showing mosaic,leaf roll symptoms and three virus-infected Hibiscus rosa-sinensis samples showing chlorosis and distortion symptoms were collected in Danzhou of Hainan Province.A pair of degenerate primers was designed according to the conserved sequence of CP gene and intergenic spacer of Whitefly-transmittal geminiviruses(WTGV),PCR product of expected size was amplified from six samples.The specific fragments were cloned and sequenced,and sequence analysis showed that PCR product had a geminiviral gene origin,which was demonstrated by BLAST.Partial DNA-A between P1 and H2 shared only 66% nucleotide sequence identity.Polygenie analysis with DNA-A of P1 and H2 from other 16 WTG,P1 was most closely related to LAYVV,H2 differed from those of other geminiviruses and constitute a new evolutionary branch.Three virus-infected Carica papaya samples showing mosaic, leaf roll symptoms and three virus-infected Hibiscus rosa-sinensis samples showing chlorosis and distortion symptoms were collected in Danzhou of Hainan Province. A pair of degenerate primers was designed according to the conserved sequence of CP gene and intergenic spacer of Whitefly-transmittal geminiviruses （WTGV）, PCR product of expected size was amplified from six samples. The specific fragments were cloned and sequenced, and sequence analysis showed that PCR product had a geminiviral gene origin,which was demonstrated by BLAST. Partial DNA-A between P1 and H2 shared only 66% nucleotide sequence identity. Polygenie analysis with DNA-A of P1 and H2 from other 16 WTG, P1 was most closely related to LAYVV,H2 differed from those of other geminiviruses and constitute a new evolutionary branch.

关 键 词：粉虱传双生病毒 海南 PCR 序列分析 

分 类 号：S436.8[农业科学—农业昆虫与害虫防治] S436.67[农业科学—植物保护]