人参皂甙Rb1对体外软骨细胞Ⅱ型胶原mRNA表达的影响  

Effects of ginsenoside Rb1 on the expression of collagen Ⅱ mRNA of chondrocytes in vitro

作　　者：王昭佩[1] 杨仁轩[1] 许树柴[1] 郭玉海[1] 邓晋丰[1] 

机构地区：[1]广州中医药大学附属广东省中医院骨科,广东广州510407

出　　处：《中国骨伤》2005年第9期543-545,共3页China Journal of Orthopaedics and Traumatology

基　　金：广东省中医药管理局资助项目(编号:102038)

摘　　要：目的:探讨兔体外软骨细胞不同代系间Ⅱ型胶原mRNA表达的差异以及人参皂甙Rb1对第2代软骨细胞Ⅱ型胶原表达的影响。方法:在成功分离培养软骨细胞后,以细胞爬片法检测原代及第2、4代软骨细胞Ⅱ型胶原mRNA表达的差异。以浓度为0·01μmol·L-1,0·1μmol·L-1,1μmol·L-1,10μmol·L-1,100μmol·L-1的Rb1作用于贴壁3d后的第2代病理软骨细胞,观察各浓度组软骨细胞Ⅱ型胶原mRNA表达情况。结果:原代、第2代、第4代软骨细胞Ⅱ型胶原mRNA表达的强度依次下降,适宜浓度的Rb1可增强第2代软骨细胞Ⅱ型胶原的表达,维持软骨细胞的正常表型。结论:兔软骨细胞不同代系间Ⅱ型胶原mRNA的表达存在一定差异,人参皂甙Rb1可能有利于第2代软骨细胞Ⅱ型胶原的表达。Objective：To study collagen Ⅱ mRNA expression of chondrocytes in different generations and the effects of ginsenoside Rbl on the expression of collagen Ⅱ mRNA of the second generation chondrocytes in rabbits. Methods：After the rabbits＇ chondrocytes were cultured successfully, the expressions of collagen Ⅱ mRNA of the first, the second and the third generation chondrocytes were detected. Different density ginsenoside Rbl, including 0.01μmol·L^-1,0.1μmol·L^-1,1μmol·L^-1,10μmol·L^-1 and 100μmol·L^-1, were exerted to the second generation chondrocyte,and then the expressions of collagen Ⅱ mRNA were observed. Results： The expression intensity of collagen Ⅱ mRNA of the first, the second and the third generation chondrocytes decreased gradually. The ginsenoside Rbl with suitable density could strengthen the expression of collagen Ⅱ mRNA,and restrain the expression of specific collagen Ⅱ .Conclusion：The expressions of collagen Ⅱ mRNA in different generation chondrocytes is different. Ginsenoside Rbl may be benefit to increase the expression of collagen Ⅱ mRNA in the second generation chondrocytes.

关 键 词：软骨细胞 基因表达 中草药 人参皂甙RB1 MRNA表达 Ⅱ型胶原 体外 胶原MRNA 人参皂甙RBL 适宜浓度 

分 类 号：R284.1[医药卫生—中药学] R734.2[医药卫生—中医学]