基因剔除小鼠研究的新进展  

The Advances in the Research of Gene Knockout Mice

作　　者：阎龙[1] 何志颖[2] 曹曙[1] 胡以平[2] 

机构地区：[1]第二军医大学研究生管理大队 [2]第二军医大学细胞生物学教研室,上海200433

出　　处：《生物技术通讯》2005年第5期560-562,共3页Letters in Biotechnology

基　　金：国家高技术发展计划项目(102-10-04-03)

摘　　要：基因剔除小鼠是活体内研究基因及蛋白质功能的理想动物模型。传统的基因剔除技术存在表型分析复杂、周期长、操作繁杂等缺点,如何更科学、更方便地建立基因剔除小鼠模型成为目前使该技术更好地应用于医学研究的关键。本文从胚胎干细胞(ES细胞)的遗传背景、组织特异性基因表达基因剔除小鼠及基因剔除技术上的革新几方面介绍了目前基因剔除小鼠研究的一些最新进展。Gene knockout mice are desirable animal model for researching the function of gene and protein in vitro. There are some disadvantages in traditional knockout technology, such as complicated analysis of phenotype, long cycle time, numerous operations, etc, so the key problem to apply this technology better to medical research at present is how to set up knockout mice model more scientifically and more conveniently. This review introduced the latest advances in the research of gene knockout mice on several aspects, which included genetic background of embryonic stem cell, tissuespecific gene expression and innovation of gene targeting technology.

关 键 词：基因剔除 胚胎干细胞 遗传背景 血管紧张扶转化酶启动子 小鼠 

分 类 号：Q78[生物学—分子生物学]