Fluorescence study on the interaction between apoCopC and cupric  

作　　者：PANG Erguo ZHAO Yaqin YANG Binsheng 

机构地区：[1]Institute of Molecular Science, Chemical Biology and Molecular Engineering Laboratory of Ministry of Education, Shanxi University, Taiyuan 030006, China

出　　处：《Chinese Science Bulletin》2005年第20期2302-2305,共4页

基　　金：supported by the National Natural Science Foundation of China(Grant No.20371031);the Natural Science Foundation of Shanxi Province(Grant No.20031017).

摘　　要：The interaction between apoCopC and cupric was investigated by fluorescence spectra, in phosphate (20 mmol/L) buffer at pH 6.0. Results suggest that the environ- ment is measured to be hydrophobic completely around tryptophan (83). At the same time, apoCopC fluorescence at 320 nm was significantly quenched with the addition of cu- pric and the 1:1 stoichiometric ratio of apoCopC to cupric was confirmed by fluorescence. In addition, the conditional binding constants were calculated to be KCu-Copc = (1.8±0.58)× 1013 mol?1 L on the basis of the results of fluorescence titra- tion curves. The apoCopC has the ability to bind specifically cupric ion.The interaction between apoCopC and cupric was investigated by fluorescence spectra, in phosphate （20 mmol/L） buffer at pH 6.0. Results suggest that the environment is measured to be hydrophobic completely around tryptophan （83）. At the same time, apoCopC fluorescence at 320 nm was significantly quenched with the addition of cupric and the 1：1 stoichiometric ratio of apoCopC to cupric was confirmed by fluorescence. In addition, the conditional binding constants were calculated to be Kcu-copc=（1.8±0.58）×10^13 mol^-1 L on the basis of the results of fluorescence titration curves. The apoCopC has the ability to bind specifically cupric ion.

关 键 词：apoCopC 荧光性 交互作用 二价铜 光谱 

分 类 号：O644.1[理学—物理化学]