程序化降温保存血管内皮功能的研究  

Study on the function of the cryopreserved vascular endothelium

作　　者：刘毅[1] 徐克森[1] 河内宽治[2] 陈雨信[1] 寿楠海[1] 

机构地区：[1]山东大学齐鲁医院普外科肝胆血管外科研究所,山东济南250012 [2]日本爱媛大学第二外科,日本松山7910295

出　　处：《中国现代普通外科进展》2005年第3期144-145,共2页Chinese Journal of Current Advances in General Surgery

摘　　要：目的探讨程序化降温对体外大鼠血管内皮的影响。方法对大鼠腹主动脉进行0.7℃/min(新鲜血管组和常规方法组)及1℃/min(试验组)程序化降温,低温保存3个月后对比观察血管内皮形态和功能变化。结果试验组的平均收缩张力及非依赖性血管舒张均较常规组高(P<0.05),但对于Ach引起的内皮依赖性血管舒张,两组差异不明显(P>0.05);光镜下试验组血管内皮细胞较常规组完整。结论改良的降温程序能够较好保持内皮的收缩功能以及非内皮依赖性舒张功能,但对内皮依赖性舒张功能效果欠佳。Objective ：We wanted to design an adequate method with which to cryopreserve rat aorta. Methods ： Rat aorta were oryopreserved at 0. 7℃/min, and kept frozen for 3 months. After being thawed, arteries were examined for contraction and vasodilation, Results ： The mean contractions to KOL,PE,ET-1 and the relaxant ratio of SNP were better preserved in Group B than in Group C（P〈0.05）. However the relaxation of Ach was badly preserved in both groups（P〉0.05）. Conclusion ： A modifiecl oryopreservation technique can better preserves endothelial contractile function and endothelium independent vasodilation of the rat aorta than the conventional method,but the enclothelium dependent vasodilation is badly preserved after the cryopreservation.

关 键 词：低温保存 内皮 血管 大鼠 Wister 

分 类 号：R654.3[医药卫生—外科学]