不同方法保存猪软骨异种移植的实验研究  

Experimental study on heterologous transplantation of pig cartilage preserved withdifferent methods

作　　者：牛扶幼[1] 陈言汤[1] 乔晨晖[1] 刘林嶓[1] 秦垦[1] 张建文[1] 陈旻静[1] 

机构地区：[1]河南医科大学第一附属医院整形外科,河南医科大学第一附属医院胸心外科

出　　处：《河南医科大学学报》1996年第1期35-40,共6页Journal of Henan Medical University

基　　金：河南省卫生厅重点资助

摘　　要：为了探索不同方法保存猪肩胛软骨对异种移植的影响，为临床应用提供科学依据。采用４种方法保存猪肩胛软骨，并按保存方法及保存时间分７组移植到日本大耳白兔背部皮下，每组５只动物，共３５只进行观察比较。①γ－射线辐射处理，４℃保存３个月为Ａ组，１个月为Ｂ组；②　（戊二醛）＝０．５％磷酸缓冲液浸泡，４℃保存３个月为Ｃ组，１个月为Ｄ组；③深低温－８０℃保存３个月为Ｅ组，１个月为Ｆ组；④新鲜软骨为Ｇ组。移植术后每周１次活体检查软骨位置及大小，术后１周、１个月、３个月、６个月，每只动物取一块软骨行大体标本和光学显微镜观察，术后６个月取软骨标本行电子显微镜观察。实验结果显示：　（戊二醛）＝０．５％浸泡、γ－射线辐射处理可降低软骨抗原性，４℃保存时间≤１个月为最佳保存时间，按软骨被吸收卒大小，依次为Ｅ、Ｆ、Ａ、Ｂ、Ｇ、Ｃ、Ｄ组。结果提示：①猪肩胛软骨可作为异种移植代用材料的最佳供体之一；②猪肩胛软骨异种移植到家兔皮下，可长期存留６个月；③本实验４种猪软骨保存方法相比较，按异种移植后软骨抗原性降低和被吸收、变形程度轻重，以　（戊二醛）＝０．５％磷酸缓冲液浸泡保存方法为最佳条件，γ－射线辐射处理次之；④　（戊二醛）＝０．５％磷酸缓冲?To research the effects of different methods of preservation on xenografts of pig scapular cartilage, we have transplanted the pig scapular cartilages preserved with 4 methods of different storage time from 1 to 3 months under the back skin of 35 Japanese white rabbits. The cartilages were divided into 7 groups according to the methods and periods of preservation. ①Group A, preserved with γ-ray irradiation at 4 ℃,for 3 months,Group B for 1 month.②Group C,preserved in 0.5% glutaraldehyde phosphate buffer at 4 ℃ for 3 months; Group D,for 1 month. ③Group E, preserved at profound hypothermia-80 ℃ for 3 months; Group F,for 1 month. ④Group G, fresh cartilage. The sites and sizes of heterografts were examined once a week after operation. Every heterograft taken from each rabbit was evaluated grossly and microscopically at 1 week, 1 month, 3 months and 6 months, respectively, after transplantation. Ultrastructural changes were observed 6 months after operation. The results indicated that the antigenicity of cartilage grafts preserved in 0.5% glutaraldehyde solution and processed with γ-ray irradiation was reduced. The optimal time for storage of cartilages was less than or equal to 1 month at 4 ℃. According to the rate of absorption of cartilage grafts,Group D, C, G, B, A, F, E were arranged at a progressive sequence. By comprehensive analysis it is concluded that: ①pig scapular cartilage is one of the optimal donors for heterograft;②pig scapular cartilage heterografts remain intact for 6 moths after being transplanted to the subcutaneous layers of rabbits; ③by comparison of the 4 methods, according to the reduction of antigenicity, and the degrees of absorption and malformation after heterografts,it is suggested that preservation of the pig cartilages in 0.5% glutaraldehyde solution may be optimal and irradiation with γ-ray may be secondary; ④preservation of pig scapular cartilage for heterografts in 0.5% glutaraldehyde solution for 1 month is the optimal condition;⑤pig scapular cartilage pr

关 键 词：软骨组织移植 异种移植 软骨 保存 

分 类 号：R687.34[医药卫生—骨科学]