CD28分子在系统性红斑狼疮患者Tc/Ts淋巴细胞上异常表达的研究  

The Abnormal Expression of CD28 in Tc/Ts Lymphocytes of the Patients with Systemic Lupus Erythematosus(SLE)

作　　者：唐江涛[1] 王兰兰[1] 刘瑾[1] 陈捷[1] 

机构地区：[1]四川大学华西医院实验医学科临床免疫实验室,四川成都610041

出　　处：《华西医学》2005年第4期661-663,共3页West China Medical Journal

摘　　要：目的:研究CD28+、CD8+CD28+、CD8+CD28-淋巴细胞在系统性红斑狼疮(SLE)患者外周血淋巴细胞中的表达情况。方法:应用CD3CD4CD8三标抗体和CD8CD28双标抗体对SLE患者淋巴细胞进行标记,用流式细胞仪测定CD3+、CD3+CD4+、CD3+CD8+、CD28+、CD8+CD28+和CD8+CD28-淋巴细胞的表达。结果:SLE患者外周血CD3+淋巴细胞明显高于健康对照组(P<0.05)。CD4/CD8T淋巴细胞比例明显倒置(P<0.01),CD3+CD8+淋巴细胞比健康对照组显著增加(P<0.01),其中以CD8+CD28-的T淋巴细胞增加为主(P<0.01),而CD8+CD28+淋巴细胞增加不明显(P>0.05),SLE患者外周血CD28+T淋巴细胞比健康对照组明显降低(P<0.05)。结论:SLE患者存在明显的T细胞功能失调。CD28+、CD8+CD28+(Tc)、CD8+CD28-(Ts)T淋巴细胞在SLE的发病机制和组织损伤中起重要作用。Objective： To study the expression of CD28^＋ , CD8^＋ CD28^＋ , CD8^＋ CD28^- lymphocytes of peripheral blood in patients with SLE. Methods： CD3^＋ , CD3^＋ CD4^＋ , CD3^＋ CD8^＋ , CD28, CD8^＋ CD28^＋和CD8^＋ CD28^- of patients with SLE were detected by a flowcytometry, after T lymphocytes were labelled with CD3CD4CD8 and CD8CD28 antibodies. Results： CD3^＋ T cells of patients with SLE were significantly higher than that of the control group （ P 〈 0.05 ）. The ratio of CD4 and CD8 lymphocytes is inverted （P 〈 0.01）. CD3^＋ CD8^＋ T cells of patients with SLE were significantly higher than that of the control group （ P 〈 0.01 ） and most of them are CD8^＋ CD28^- T lymphocytes （ P 〈 0.01 ）, while the change of CD8^ ＋ CD28^ ＋ lymphocytes is not obvious （ P 〉 0.05 ）. CD28^＋ T cells of patients with SLE were significantly lower than that of the control group （ P 〈 0.05）. Conclusion： There was a obvious ruction disorder of T lymphocytes in patients with SLE. CD28^＋ , CD8^＋ CD28^＋ （Tc）, CD8^＋ CD28^- （Ts） T lymphocytes may play an important role in the mechanism and tissue damage of SLE.

关 键 词：系统性红斑狼疮 CD3CD4CD8 CD8CD28 流式细胞仪 

分 类 号：R593.241[医药卫生—内科学]