星形胶质细胞毒蕈碱样乙酰胆碱受体M_2、M_4亚型基因克隆及序列分析  

Sequence analysis of muscarinic receptors M_2 and M_4 expression by cultured rat astrocyte cells

作　　者：张晓娟[1] 汪海[1] 

机构地区：[1]军事医学科学院毒物药物研究所,北京100850

出　　处：《中国临床药理学与治疗学》2005年第10期1108-1111,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：国家自然科学基金(№30371641)军事医学科学院创新基金资助

摘　　要：目的：克隆胶质细胞M_2、M_4受体亚型基因序列，并比较胶质细胞M_2、M_4受体亚型基因序列和蛋白质序列与神经元细胞M_2、M_4受体基因序列和蛋白质序列间的差异。方法：根据神经元细胞M_2、M_4受体基因序列设计出针对M_2、M_4受体基因序列全长的特异性探针，采用RT-PCR方法扩增胶质细胞M_2、M_4受体亚型基因序列，并对其进行克隆测序。结果：通过RT-PCR方法扩增胶质细胞M_2、M_4受体亚型基因序列，与神经元细胞M_2、M_4受体比较，M_2受体差异碱基17个，发生氨基酸改变的有8个；M_4受体差异碱基3个，发生氨基酸改变的有2个。结论：胶质细胞M_2、M_4受体与神经细胞M_2、M_4受体亚型在基因序列和氨基酸序列上具有明显差异。AIM： To clone the muscarinic receptor M2, M4 sequence of astrocyte cell, and compare the gene and protein sequence with those of neuron. METHODS： The specific primers were designed to clone the M2 and M4 sequence of astrocyte cell according to the sequences that of neuron by RT-PCR, and then the sequences were sequenced. RESHLTS AND CONCLUSIONS： The M2 and M4 sequences expression were compared by astrocyte cells and neurons, and the difference was marked. The differences of the gene and protein sequences are evident of M2 and M4 between astrocyte cell and neuron.

关 键 词：非神经性胆碱系统 星形胶质细胞 基因克隆 聚合酶链式反应 序列分析 M受体 

分 类 号：R965.1[医药卫生—药理学]