丹参对糖尿病大鼠肾脏的保护作用及其机制研究  

Protective effects of Salvia miltiorrhiza on rats with streptozotocin diabetes and its mechanism

作　　者：柳刚[1] 关广聚[1] 亓同钢[2] 傅余芹[1] 李学刚[1] 孙云[1] 吴涛[1] 文蓉珠[1] 

机构地区：[1]山东大学第二医院肾内科,山东济南250033 [2]山东大学第二医院分子生物学实验室,山东济南250033

出　　处：《中西医结合学报》2005年第6期459-462,共4页Journal of Chinese Integrative Medicine

基　　金：国家教育部博士点基金资助项目(No.20030422068);山东省中医药管理局资助项目(No.200329105)

摘　　要：目的:探讨丹参对糖尿病大鼠肾脏的保护作用及其机制。方法:采用单侧肾切除、腹腔注射链脲佐菌素诱导糖尿病肾病大鼠模型,予以丹参药物干预。观察大鼠肾脏形态学及肾功能的变化。采用荧光实时定量逆转录-聚合酶链反应法检测转化生长因子β1(transforming growth factor-beta1,TGF-β1)、结缔组织生长因子(connective tissue growth factor,CTGF)、纤溶酶原激活物抑制物1(plasminogen activator inhibi-tor-1,PAI-1)等细胞因子在糖尿病大鼠肾皮质中的表达水平。结果:与正常对照组比较,用药第8周末糖尿病肾病模型组大鼠的肾脏肥大指数、平均肾小球体积、尿白蛋白排泄率、肌酐清除率均有明显升高(P<0.05),肾皮质TGF-β1、CTGF、PAI-1和纤维连接蛋白的表达水平也有显著增高(P<0.05)。丹参治疗组大鼠的上述指标与糖尿病肾病模型组比较,则有明显的降低(P<0.05)。结论:丹参可通过抑制TGF-β1、CTGF、PAI-1等细胞因子的表达,从而对糖尿病大鼠肾脏起保护作用。Objective： To explore the effects of Salvia miltiorrhiza on renal morphology and renal function of rats with streptozotocin diabetes. Methods： Thirty male Wistar rats were randomly divided into three groups, which were normal control group, untreated group and Salvia rniltiorrhiza-treated group. Diabetic nephropathy was induced in rats of the last two groups by intraperitoneal injection of streptozotocin after unilateral nephrectomy. Then the rats in the normal control and untreated groups were fed with normal saline while those in the Salvia miltiorrhiza-treated group were fed Salvia miltiorrhiza preparation for 8 weeks. The glomerular volume （VG）, kidney-to-body weight ratio （KW/BW）, urinary albumin excretion rate （UAER） and creatinine clearance （Ccr） were observed. The expression levels of transforming growth factor-beta1 （TGF- β1 ）, connective tissue growth factor （CTGF）, fibronectin （FN） and plasminogen activator inhibitor-1 （PAl-l） were detected by real-time quantitative reverse transcriptase-polymerase chain reaction （RT-PCR） at the end of the experiment. Results： UAER, Ccr, VG and KW/BW ratio were significantly higher in the untreated group than those in the normal control group （P^0.05）. The expression levels of TGF-β1, CTGF, PAI-1 and FN in the untreated group were also significantly higher as compared with those in the normal control group （P〈0.05）. UAER, Ccr, VG, KW/BW ratio and the levels of TGF-β1, CTGF, PAI-1 and FN in the Salvia miltiorrhiza-treated group were obviously lower than those in the untreated group （P〈0.05）. Conclusion： Salvia miltiorrhiza can protect rats with streptozotocin diabetes from diabetic nephropathy by suppressing the over-expressions of TGF-β1, CTGF, PAI-1 and FN in renal cortex.

关 键 词：链脲霉素糖尿病 丹参 转化生长因子Β1 结缔组织生长因子 纤溶酶激活物抑制物1 

分 类 号：R285.5[医药卫生—中药学]