生脉注射液对5-FU增效减毒作用的实验研究  

Experimental study on effects of Shengmai Injection: enhancing 5-FU anti-tumor efficacy and reducing its toxicity

作　　者：陈震[1] 王鹏[1] 黄雯霞[1] 刘鲁明[1] 

机构地区：[1]复旦大学肿瘤医院中医科,上海200032

出　　处：《中西医结合学报》2005年第6期476-479,共4页Journal of Chinese Integrative Medicine

基　　金：上海市科委基金资助项目(No.03DZ19501)

摘　　要：目的:观察生脉注射液对5-氟尿嘧啶(5-fluorouracil,5-FU)抗肿瘤的增效减毒作用。方法:建立H22肝癌荷瘤鼠模型后,将50只小鼠随机分为5组:对照组、5-FU组和生脉注射液(大、中、小剂量)联合5-FU组,每组10只。造模次日开始进行干预处理:对照组、5-FU组和生脉注射液(大、中、小剂量)联合5-FU组分别腹腔注射等容量生理盐水、5-FU、生脉注射液(大、中、小剂量)联合5-FU,共14 d。停药次日处死小鼠,观察瘤重抑制率、免疫功能、肝肾功能及外周血细胞变化。结果:生脉注射液联合5-FU各组小鼠的肿瘤生长抑制率明显高于5-FU组和对照组(P<0.05);与对照组相比,5-FU组CD3、CD4、CD4/CD8、IgGI、gM值明显降低(P<0.05),而各生脉注射液联合5-FU组CD3、CD4、CD4/CD8I、gGI、gM值升高(P<0.05);与对照组相比,5-FU组的血丙氨酸氨基转移酶升高、白细胞和血小板数量降低(P<0.05);而各生脉注射液联合5-FU组血丙氨酸氨基转移酶与对照组比较无明显变化,白细胞数下降程度较轻。结论:生脉注射液可增加5-FU的抑瘤效果,提高机体免疫功能,减轻化疗毒副反应。Objevtive： To observe the effects of Shengmai Injection on enhancing efficacy and reducing toxicity of 5-fluorouracil （5-FU）. Metheds： Fifty hepatoma 22 bearing mice were randomly divided into five groups： control group, 5-Fu group, Shengmai Injection （low, medium and high dose） combined with 5-FU groups. There were 10 mice in each group. Mice in the five groups were injected introperitoneally the same amount of normal saline, 5-FU （20 mg ·kg^-1· d^-1） and Shengmai Injection （3. 5 ml ·kg^-1· d^-1, 7 ml·kg^-1· d^-1 and 14 ml ·kg^-1· d^-1） combined with 5-FU respectively, once a day for 14 days. After that, all mice were killed and the tumor inhibiting rates, index of immunological function, liver and kidney function and the blood cells in the peripheral blood were observed. Results： The tumor inhibiting rates were higher in each Shengmai Injection combined with 5-FU group than that in the 5-FU group （P〈0.05）. The levels of CD3,CD4,CD4/CD8,1gG,IgM in 5-FU group were lower （ P〈0.05）, while those in the three Shengmai Injection combined with 5-FU groups were higher than those in the control group （P〈0.05）. The level of serum alanine aminotransferase （ALT） was higher and the WBC and PLT counts in the peripheral blood were lower in 5-FU group than those in the control group （ P〈 0.05）. But the levels of serum ALT in the three Shengmai Injection combined with 5-FU groups were consistent with that in the control group and the amounts of WBC decreased slightly. Conclusion： Shengmai Injection can enhance the anti-tumor effect of 5-FU. It can also improve the immunological function and reduce the adverse reactions of chemotherapy.

关 键 词：抑瘤率 免疫力 副反应 5-氟尿嘧啶 生脉注射液 

分 类 号：R285[医药卫生—中药学]