湖南省石杉属植物中石杉碱甲含量的研究  

Study on content of huperzine A in six plants of Huperzia from Hunan province

作　　者：王峻[1] 潘胜利[1] 

机构地区：[1]复旦大学药学院,上海200032

出　　处：《中国药学杂志》2005年第21期1616-1618,共3页Chinese Pharmaceutical Journal

摘　　要：目的测定湖南产6种(含一新种)石杉属植物中石杉碱甲含量,为科学利用该植物提供可靠依据。方法于该省野外实地采集石杉属植物,以2%酒石酸溶液提取总碱,用改进的高效液相色谱法测定石杉碱甲的含量(外标法)。结果所采集6种石杉属植物中,石杉碱甲在蛇足石杉、狭叶石杉、长柄石杉、四川石杉、湖南石杉(拟新种)中均有分布,长柄石杉中含量最高,达0.0789%;本实验证明皱边石杉中不含石杉碱甲,先前两篇高含量报道应为鉴定之误。结论本测定方法简便、准确;在该属中4种形态极难区分的种之间,石杉碱甲含量具有分类学意义。OBJECTIVE To determine the content of huperzine A in six plants （including a novel species） of Genus Huperzia Bernh. from Hunan province.METHODS Plants were collected in the wild field in Hunan province. Total alkaloids were extracted by 2% tattle acid, and were analyzed by an improved HPLC. The content of huperzine A was determined by external standards. RESULTS Six species from Huperz~ Bemh. were gathered. Huperzine A was found in H. serrata, H. serrata var. intermedia, H. sutchueniana, H. hunaniana and especially in H. javanica, whose content reach up to 0.078 9%. The content of huperzine A in H. crispata was not ohserved. CONCLUSION The method is simple and accurate. The content of huperzine A is an aidant taxonomic character in four species that are hard to be morphologically differentiated from each other.

关 键 词：石杉碱甲 石杉属 高效液相色谱法 

分 类 号：R931.5[医药卫生—生药学]