生防菌ZJY-1及ZJY-116的GFP标记及其在黄瓜根围的生态适应性  

Labeling of biocontrol agents ZJY-1 and ZJY-116 gfp gene and its ecological adaptability in cucumber rhizosphere

作　　者：张昕[1] 张炳欣[1] 喻景权[1] 张震[2] 沈卫峰[2] 陈振宇[3] 石江[4] 

机构地区：[1]浙江大学农业与生物技术学院,杭州310029 [2]浙江农业科学院,杭州310021 [3]浙江大学图书馆,杭州310029 [4]杭州市蔬菜研究所,杭州316004

出　　处：《应用生态学报》2005年第11期2144-2148,共5页Chinese Journal of Applied Ecology

基　　金：国家自然科学基金重点资助项目(30230250).

摘　　要：将具有绿色荧光蛋白(GFP)标记和氯霉素抗性的重组质粒pRP22-GFP导入生防菌BrevibacillusbrevisZJY-1和Bacillus subtilisZJY-116中,用这些标记菌株处理黄瓜种子,出苗后通过定期分离计数具有上述表型的转化子,研究了2株生防菌在黄瓜根围的定殖规律.结果表明,在整个生育期2株生防菌株均能在黄瓜根围有效定殖,并在黄瓜盛花期和盛果期出现数量高峰.盆栽试验发现,引入黄瓜根围的2株生防菌有向周边杂草迁移的特性,且在前一季寄主植物死亡后,菌株可在下一季植株根围增殖.The recombined plasmid pRP22-GFP contained with gfp gene and chloramphenicol resistant was successfully introduced into two biocontrol agents BrevibaciUus brevis ZJY-1 and Bacillus subtilis ZJY-116. After seed inoculation, the survival and colonization of the two strains were studied by periodically retrieving the GFP-tagged strains in the cucumber rhizosphere based on the selective markers. The results showed that both the strains could successfully colonize in the rhizosphere during the whole life of cucumber, and a higher colonization level was observed during anthesis and fruition stages. In pot trials, they could migrate to the nearby non-inoculated spontaneous weed plants, and reestablish in the rhizosphere of plants subsequently grown in the same pot.

关 键 词：质粒pRP22-GFP GFP基因 短短芽孢杆菌 枯草芽孢杆菌 黄瓜根围 定殖 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]