氟化钠对人胚支气管上皮细胞肿瘤标志物的影响  

The Effect of Sodium Fluoride on Tumor Markers of Human Embryonic Bronchial Epithelium Cells

作　　者：马道新[1] 刘春生[1] 李杰[2] 张晓梅[3] 于书彦[3] 孙建霞[2] 

机构地区：[1]山东大学齐鲁医院血液学研究室,山东济南250012 [2]山东大学公共卫生学院,山东济南250012 [3]山东大学医学院,山东济南250012

出　　处：《环境与健康杂志》2005年第6期403-405,F0003,共4页Journal of Environment and Health

基　　金：国家自然科学基金资助项目(39670624)

摘　　要：目的探讨氟化钠(NaF)对人胚支气管上皮(HEBE)细胞肿瘤标志物改变的影响。方法无菌分离人胚支气管上皮细胞,传代培养,经不同浓度的NaF(0、1、2、4、6mmol/L)暴露36h,换液后继续培养36h,利用四甲基偶氮唑蓝(MTT)法检测NaF对HEBE细胞的毒性,同时收集培养液上清液,用酶联免疫分析方法(ELISA)检测癌胚抗原(CEA)、神经元特异性烯醇化酶(NSE)、角质蛋白21鄄1(CYFRA21-1)、细胞角蛋白19(CK鄄19)等肺癌肿瘤标志物。结果NaF作用后细胞出现毒性反应,脱落皱缩,胞内颗粒增多,且有剂量依赖性,当NaF为6mmol/L时,吸光度(A)值仅为0.15±0.07,几乎无存活细胞;对照组及各NaF染毒组肺癌肿瘤标志物水平均较低,且差异无统计学意义。结论NaF可引起人胚支气管上皮细胞的细胞毒性,但尚未见其可引起肺癌肿瘤标志物的改变。Objective To explore the effect of sodium fluoride （NaF） on the tumor markers of human embryonic bronchial epitllelium （HEBE） cells. Methods HEBE cells were collected from the human abortive fetues. The cells were exposed to NaF of several concentrations for 36 h. After rinsed, the cells were incubated for 36 h again. The NaF toxicity to HEBE cells was detected using MTT method. The supernatant was collected and the lung tumor markers were detected using ELISA method. Results NaF was toxic to the HEBE cells, and the toxicity was increased with the NaF doses. There were few survival HEBE cells at 6 mmol/L NaF. The tumor markers in both of the control and experiment groups were very low, and no significant difference had been seen between them. Conclusion NaF may damage HEBE cells, but may not influence the tumor markers of HEBE cells.

关 键 词：氟化钠 人胚支气管上皮细胞 肿瘤标志物 

分 类 号：R994.6[医药卫生—毒理学]