甲醛暴露的医学生口腔黏膜细胞的DNA-蛋白质交联  

Effect of Air Formaldehyde Exposure of Medical Students on DNA-Protein Crosslinks in Their Buccal Mucosa Cells

作　　者：颜进[1] 刘英帅[2] 迟欣[1] 鲁志松[2] 石玉琴[1] 杨旭[2] 张本延[1] 

机构地区：[1]武汉科技大学医学院预防医学系,湖北武汉430080 [2]华中师范大学生命科学院,湖北武汉430079

出　　处：《环境与健康杂志》2005年第6期422-424,共3页Journal of Environment and Health

摘　　要：目的研究医学生上解剖课甲醛暴露后对口腔黏膜DNA鄄蛋白质交联(DPC)的影响。方法以37名(男20名,女17名)正在上解剖课程的医学院学生和40名(男20名,女20名)同年级理学院的学生为研究对象。取口腔黏膜上皮细胞,应用改良的SDS鄄KCl沉淀法,测定口腔黏膜细胞DNA鄄蛋白质交联率。结果两组学生性别和年龄构成差异无统计学意义。解剖实验室空气甲醛浓度范围在0.42～1.57mg/m3之间。医学院学生口腔黏膜细胞DNA鄄蛋白质交联率(25.72%±6.48%)高于对照组(22.88%±5.34%),P<0.05。其中医学院女生DNA鄄蛋白质交联率(27.72%±5.76%)明显高于对照组女生(22.29%±4.20%),P<0.01。结论医学生解剖课甲醛暴露可导致口腔黏膜细胞DNA鄄蛋白质交联率增高。女性对气态甲醛暴露可能较男性敏感。Objective To study the effect of formaldehyde exposure on DNA-protein crosslinks （DPC） in buccal mucosa cells in students who were taking anatomy course. Methods The modified SDS-KCl precipitation assay published by Zhitkovich and Costa in 1992 was applied to detect DNA-protein crosslinks in human cells. And this method has been used to explore DPC induced by different pollutants in numerous studies. 37 medical students （20 males and 17 females） aged 19.24±1.09 （mean±standard deviation） and 40 students （20 males and 20 females） in natural science college aged 19.55±0.99 （mean±standard deviation） were studied. The frequency of exposure to formaldehyde was 6 h per week in exposed group. Results Concentrations of formaldehyde in anatomy laboratory ranged from 0.42 to 1.57 mg/m^3. Exposure to formaldehyde resulted in an increase of DNAprotein crosslinks. The percentage （mean±standard deviation） of DNA-protein crosslinks in exposed and nonexposed students were 25.72%±6.48% and 22.88%±5.34% respectively（P〈0.05）. Formaldehyde caused a significant increase in DNA-protein crosslinks of the females in two groups （27.72%±5.76% and 22.29%±4.20%, P〈0.01）. However, there was no difference in males （24.02%± 6.72% and 23.48%±6.33%, P〉0.05）. Conclusion The result of the present study suggests that formaldehyde exposure in medical students increases the frequency of DNA-protein crosslinks in buccal mucosa cells and females may be more sensitive to formaldehyde exposure.

关 键 词：甲醛 DNA-蛋白质交联 学生 口腔黏膜细胞 

分 类 号：R181.3[医药卫生—流行病学]