甲醛诱导小鼠骨髓细胞微核和DNA损伤的研究  

A Study on Genetic Toxicity of Formaldehyde

作　　者：戎芬[1] 原福胜[1] 赵五红[1] 白剑英[1] 史晓丽[1] 

机构地区：[1]山西医科大学公共卫生学院环境卫生教研室,山西太原030001

出　　处：《环境与健康杂志》2005年第6期425-426,共2页Journal of Environment and Health

基　　金：山西省自然科学基金资助项目(20031098)

摘　　要：目的研究甲醛对小鼠的遗传损伤作用。方法选择昆明种小鼠30只,随机分为5组,用分析纯甲醛配制成0、1.25、2.50、5.00mg/m3浓度,对小鼠进行静式吸入染毒,2h/d,连续15d,阳性对照组腹腔注射环磷酰胺40mg/kg,每天1次,连续2d。采用微核试验和彗星试验检测甲醛的遗传毒性。结果甲醛能引起小鼠骨髓细胞微核率升高和外周血淋巴细胞DNA不同程度的电泳迁移,与对照组比较,差异有统计学意义(P<0.05)。微核率随着甲醛剂量的增加而升高;迁移率和迁移度在1.25mg/m3剂量组与2.50、5.00mg/m3组之间差异有统计学意义,2.50mg/m3与5.00mg/m3组之间差异无统计学意义。结论甲醛对小鼠有遗传损伤作用,随着甲醛剂量的增加,损伤加重。Objective To explore the genotoxicity of formaldehyde to mice. Methods Mice were exposed to formaldehyde of several concentrations（1.25, 0.50, and 5.00 mg/m^3） in the toxicant exposure chamber, 2 h/d for 15 consecutive days. Bone marrow micronuclei test and single cell gel electrophoresis were employed to test the genotoxicity. Results Compared with the control group, a significant increase in the rate of micronuclei and the DNA damage of peripheral lymphocytes in experimental groups were found. As to the rate ofmicronuclei and DNA damage, an obvious dose-effect relationship was showed. Conclusion Formaldehyde has a genotoxic effect for mice, much more attention should be paid in this research field.

关 键 词：甲醛 DNA损伤 微核试验 单细胞凝胶电泳 

分 类 号：R994.6[医药卫生—毒理学]