BMP-2基因给药修复实验性羊股骨头坏死  

Repairing experimental femoral head necrosis of goat with BMP-2 gene medicine

作　　者：陆斌[1] 汤亭亭[1] 岳冰[1] 戴尅戎[1] 楼觉人[2] 

机构地区：[1]上海第二医科大学附属第九人民医院骨科 [2]美国华盛顿大学医学院骨科

出　　处：《中华骨科杂志》2005年第10期603-607,共5页Chinese Journal of Orthopaedics

基　　金：上海市科委重点课题(024119016)

摘　　要：目的利用人BMP-2基因转染的自体骨髓间充质干细胞复合多孔β-磷酸三钙材料治疗股骨头坏死。方法对22只羊建立股骨头缺血性坏死模型。未治疗组4只;治疗组18只于3周后刮除坏死骨组织,右侧植入BMP-2基因转染的自体骨髓间充质干细胞和多孔磷酸三钙复合物(BMP-2组),左侧植入β-半乳糖苷酶(β-gal,作为对照基因)基因转染的自体骨髓间充质干细胞和多孔磷酸三钙复合物(β-gal组)。植入后第1、2、3周用ELISA方法测定植入局部的BMP-2蛋白表达水平。植入前、植入后6、10、16周取股骨头标本,观察骨长入情况,并对16周标本行骨形态计量学分析和影像学观察。未治疗组动物于造模后16周摄Χ线片行影像学观察。结果植入后1、2、3周BMP-2组在股骨头局部有BMP-2蛋白的高表达,明显高于β-gal组。植入前软骨下骨部位出现大量空骨陷窝,骨髓腔纤维化。植入后6、10、16周BMP-2组均有明显成骨,16周时坏死区被完全修复;β-gal组只有较少新骨形成,16周时坏死区仍多为纤维组织。BMP-2组新骨体积明显大于β-gal组。造模后16周未治疗组双侧股骨头塌陷;植入后16周β-gal组股骨头存在囊状密度减低区;BMP-2组股骨头形态规则,无塌陷。结论BMP-2基因转染的骨髓间充质干细胞与多孔β-磷酸三钙复合后可修复早期股骨头坏死。Objective To repair the experimental femoral head necrosis with the implantation of triealcium phosphate （TCP） loaded with hulnan bone morphogenetic protein-2 （hBMP-2） gene transfeeted bone marrow derived mesenehvmal stenl cells （BMSCs）. Methods Established the anilnal models of femoral head necrosis in 22 goats bv the ligation of the lateral and medial circumflex femoral arteries and delivery of liquid nitrogen into femoral head. 4 goats served as the control group without any treatment. 3 weeks after surgert, necrotic bone in the other 18 goats were removed and TCP loaded gene transfer cells were implanted into the femoral head with BMP-2 gelle transfected BMSCs in right side and β-gal gene transfected BMSCs in left side. BMP-2 concentrations in felnoral head were tested by the ELISA at 1st, 2nd, 3rd week after implantation. Histologieal observation was done before and at 6th. 10th, 16th week after implantation. New bone volumes （NBV） were measured at 16th week after implantation bv the histomorphometry method. X-ray was taken at 16th week after operation in untreated group and after treatment in BMP-2 group and β-gal group. Results The concentration of BMP-2 in femoral head of BMP-2 group was higher than that of β-gal group at different time point. Empty lacunae and fibrotic marrow were demonstrated before implantation. New bone was evident in the implantation fiehl of BMP-2 group while fibrous tissues were evident in that of β-gal group at 6th, lOth and 16th week after treatment. Histomnrphology analysis indicated that the NBV in BMP- 2 group was significantly higher than that in β-gal group. Articular surface collapse were observed in the X- ray photograph of untreated group. Regular shape and normal density of femoral head in BMP-2 group compared with the irregular shape and low density of femoral head in β-gal group could he demonstrated in the X-ray photograph 16 weeks after treatment. Conclusion Implantation of the TCP loaded with human BMP-2 gene transfected BMSCs could repair earl

关 键 词：股骨头坏死 山羊 间质干细胞 骨形态发生蛋白类 磷酸钙类 BMP-2基因给药 早期股骨头坏死 自体骨髓间充质干细胞 修复 多孔磷酸三钙 实验性 β-gal 股骨头缺血性坏死 β-磷酸三钙 

分 类 号：R681.8[医药卫生—骨科学]